Molecular prevalence and genetic characterization of Enterocytozoon bieneusi in cattle in a dairy farm in Türkiye.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2025-03-31 DOI:10.1186/s12917-025-04701-3

Tülay Öncü Öner, Hüseyin Can, Aysu Değirmenci Döşkaya, Mervenur Güvendi, Sedef Erkunt Alak, Ecem Sürgeç, Cemal Ün, Mert Döşkaya, Adnan Yüksel Gürüz, Muhammet Karakavuk

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引用次数: 0

Abstract

Background: Microsporidia, which are unicellular and obligate intracellular eukaryotic pathogens, can infect many animal species and humans. Cattle are known to be an important source for the spread of Enterocytozoon bieneusi (E. bieneusi) to humans and animals. Therefore, this study aimed to investigate E. bieneusi in fecal samples collected from cattle and environmental samples within a dairy farm and to genotype E. bieneusi-positive samples. For this purpose, a Nested PCR targeting a region of the E. bieneusi ribosomal DNA internal transcribed spacer (ITS) was applied to the DNA samples extracted from fecal (n = 47) or environmental samples (n = 41). Later, sequencing data belonging to E. bieneusi-positive samples were analyzed by BLAST and phylogenetic analysis for genotyping.

Results: Nested PCR screening detected 15 E. bieneusi-positive samples and among them six (12.8%; 6/47) were detected in fecal samples while nine (21.9%; 9/41) were detected in environmental samples (including drinking water containers, mangers, feeding bottle, milk heating tank and towel). When cattle were categorized by their age, the molecular prevalence of E. bieneusi for cattle < 2 months old was detected as 19.2% (5/26). However, in cattle group > 2 months old, the molecular prevalence of E. bieneusi was 10% (1/10). Surprisingly, remaining cattle > 2 years old (n = 11) were found to be E. bieneusi-negative. BLAST and phylogenetic analyses revealed type IV as well as 11 new genotypes designated as NG1 to NG11.

Conclusion: These findings point out that cattle and farms are important sources for the transmission of E. bieneusi spores to humans or animals.

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浙江省某奶牛场牛双氏肠细胞虫分子流行病学及遗传特征分析。

背景：微孢子虫是一种单细胞和专性细胞内真核病原体，可感染多种动物和人类。众所周知，牛是比氏肠胞虫（比氏肠胞虫）向人类和动物传播的重要来源。因此，本研究旨在调查从奶牛粪便样本和奶牛场环境样本中收集的白氏埃希氏菌，并对白氏埃希氏菌阳性样本进行基因分型。为此，我们对粪便（n = 47）或环境样本（n = 41）中提取的DNA样本采用了巢式PCR方法，该方法针对的是bieneusi核糖体DNA内转录间隔（ITS）的一个区域。随后，利用BLAST和系统发育分析对阳性样本的测序数据进行基因分型分析。结果：巢式PCR筛选检出15份阳性标本，其中6份(12.8%；6/47)， 9例(21.9%；环境样品（包括饮用水容器、管理器、喂养瓶、牛奶加热罐和毛巾）中检测到9/41)。按牛龄分类时，2月龄牛的比氏埃希菌分子感染率为10%（1/10）。令人惊讶的是，剩下的0 ~ 2岁的牛（n = 11）被发现为布氏大肠杆菌阴性。BLAST和系统发育分析发现了IV型和11个新的基因型，命名为NG1至NG11。结论：这些发现表明，牛和农场是布氏芽孢杆菌传播给人类或动物的重要来源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.