Supplying silicon reduces cadmium accumulation in pak choi by decreasing soil Cd bioavailability and altering the microbial community.

Abstract

Silicon-containing materials have been widely used in Cd-contaminated soil remediation. However, the immobilization effects of sodium silicate on Cd migration and transformation in an acidic soil-vegetable system have not been thoroughly studied. Herein, a pot experiment was performed to investigate the effects of sodium silicate application on pak choi growth, oxidative status, Cd uptake and accumulation in pak choi, soil Cd bioavailability and fractions, and soil bacterial communities. The results showed that sodium silicate application significantly increased soil pH (0.29-1.61 units) and induced the transformation of the Cd fraction from an exchangeable fraction (Exc-Cd) into an iron and manganese oxide-bound fraction (OX-Cd) and organic matter-bound fraction (OM-Cd), decreasing Cd bioavailability by 13.7-20.8% in Cd-contaminated acidic soil. As a result, sodium silicate application significantly alleviated Cd toxicity, enhanced pak choi growth, and reduced Cd concentration in roots by 23.5-89.0% and in shoots by 58.5-81.0%, with Cd concentration in the edible part at a Si application rate equal to or greater than 0.4 g Si per kg soil falling below the safety limits for Cd as defined in China's food safety standard (GB 2762-2022). In addition, sodium silicate application significantly increased soil bacterial richness (Ace index and Chao1) and diversity (Shannon and Simpson index) and altered the soil microbial structure. These findings suggested that sodium silicate has great potential as an environmentally friendly amendment to immobilize Cd-contaminated acidic soil and reduce Cd accumulation in vegetables.