Improving experimental reproducibility through comprehensive research protocols

IF 2.8 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Ivana Novak
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引用次数: 0

Abstract

With this “In the Limelight: Research Protocols” special issue, FEBS Open Bio aims to highlight the critical importance of publishing reproducible and detailed scientific protocols that can be broadly adopted by laboratories working in molecular and cellular life sciences. This collection includes four protocols focused on sample preparation for structural analysis of macromolecules using X-ray crystallography, covering both in vitro and in vivo approaches. Two additional protocols demonstrate the use of cellular systems to screen the enzymatic activity of various proteins, with potential applications in high-throughput screening. Another protocol provides a comprehensive guide for the preparation and analysis of selective autophagy flux in cultured cells using flow cytometry. Finally, the issue concludes with a protocol integrating classical behavioral tests with cognitive components to assess both physical and cognitive dimensions of frailty.

通过全面的研究方案提高实验的可重复性
通过这一期“引人注目:研究方案”特刊,FEBS Open Bio旨在强调发表可复制和详细的科学方案的重要性,这些方案可以被从事分子和细胞生命科学的实验室广泛采用。该系列包括四种方案,重点是使用x射线晶体学进行大分子结构分析的样品制备,涵盖体外和体内方法。另外两个方案展示了使用细胞系统筛选各种蛋白质的酶活性,具有高通量筛选的潜在应用。另一种方案为流式细胞术制备和分析培养细胞的选择性自噬通量提供了全面的指导。最后,本文总结了一个将经典行为测试与认知成分结合起来的方案,以评估身体和认知方面的脆弱性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
FEBS Open Bio
FEBS Open Bio BIOCHEMISTRY & MOLECULAR BIOLOGY-
CiteScore
5.10
自引率
0.00%
发文量
173
审稿时长
10 weeks
期刊介绍: FEBS Open Bio is an online-only open access journal for the rapid publication of research articles in molecular and cellular life sciences in both health and disease. The journal''s peer review process focuses on the technical soundness of papers, leaving the assessment of their impact and importance to the scientific community. FEBS Open Bio is owned by the Federation of European Biochemical Societies (FEBS), a not-for-profit organization, and is published on behalf of FEBS by FEBS Press and Wiley. Any income from the journal will be used to support scientists through fellowships, courses, travel grants, prizes and other FEBS initiatives.
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