Investigation of Curcumin-β-Cyclodextrin Complex Release in Injectable Hyaluronic Acid/Quince Seed Gum Hydrogel

IF 3.4 4区 医学 Q2 PHARMACOLOGY & PHARMACY
Serdar Kolay, Nilhan Kayaman Apohan, Erdinç Babuç, Gökay Gün
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引用次数: 0

Abstract

Injectable hydrogels play a crucial role in various biomedical applications, serving as fillers in tissue engineering, facilitating cell repair, and contributing to the development of drug delivery systems. This study aims to investigate the release of dissolved curcumin from a new injectable hyaluronic acid/quince seed gum (HA/QSG) hydrogel. Curcumin possesses numerous biological activities, including anti-cancer, antioxidant, antimicrobial, and anti-inflammatory properties. However, due to its very low water solubility, its bioavailability is poor. To address this, curcumin was encapsulated in three inclusion complexes: Cur 1:2 β-CD, Cur 1:4 β-CD, and Cur 1:6 β-CD. These Cur-β-CD inclusion complexes were lyophilized and converted into a water-soluble form. The curcumin, bisdemethoxycurcumin, and desmethoxycurcumin content of the obtained lyophilized Cur-β-CD complexes were analyzed using the HPLC method. HA-QSG hydrogels were loaded with Cur 1:2 β-CD inclusion complex in compositions of 0.75% (w/w), 0.50% (w/w), and 0.25% (w/w). The dissolution profiles of the HA-QSG hydrogels were examined in a pH 6.8 phosphate buffer medium, used as the swelling medium in intra-articular hydrogels. The initial burst of the 0.75% (w/w) hydrogel reached 9% release within the first 15 min, whereas the 0.25% (w/w) hydrogel exhibited only 6% release during the same period. The 0.75%(w/w) and 0.50% (w/w) hydrogels displayed very similar dissolution profiles, with a slightly faster release in the early stages of dissolutions, compared to the 0.25%(w/w) Cur β-CD-HA-QSG hydrogel. The 0.25%(w/w) Cur β-CD-HA-QSG hydrogel demonstrated a relatively slower release rate particularly during the initial stage of dissolution period. However, all three formulations reached approximately 98% release within 24 h. While the 0.75%(w/w) and 0.50%(w/w) curcumin-loaded HA-QSG hydrogels, with their rapid initial release, may be suited for intra-articular applications requiring quick drug availability, the 0.25%(w/w) curcumin-loaded HA-QSG hydrogel, with its slower release, may be more beneficial for sustained intra-articular delivery.

Graphical Abstract

姜黄素-β-环糊精复合物在透明质酸/木瓜籽胶注射凝胶中的释放研究
可注射水凝胶在各种生物医学应用中发挥着至关重要的作用,在组织工程中充当填充物,促进细胞修复,并有助于药物输送系统的发展。本研究旨在研究一种新型透明质酸/木瓜籽胶(HA/QSG)注射水凝胶中可溶性姜黄素的释放。姜黄素具有多种生物活性,包括抗癌、抗氧化、抗菌和抗炎等特性。但由于其水溶性极低,生物利用度较差。为了解决这个问题,我们将姜黄素包被在三种包合物中:Cur 1:2 β-CD、Cur 1:4 β-CD和Cur 1:6 β-CD。这些Cur-β-CD包合物被冻干并转化为水溶性形式。用高效液相色谱法分析得到的冻干Cur-β-CD配合物中姜黄素、双去甲氧基姜黄素和去甲氧基姜黄素的含量。HA-QSG水凝胶分别以0.75% (w/w)、0.50% (w/w)和0.25% (w/w)的比例负载Cur 1:2 β-CD包合物。HA-QSG水凝胶在pH 6.8的磷酸盐缓冲介质中溶解,作为关节内水凝胶的溶胀介质。0.75% (w/w)的水凝胶在15分钟内释放量达到9%,而0.25% (w/w)的水凝胶在同一时间内释放量仅为6%。0.75%(w/w)和0.50% (w/w)的水凝胶在溶解初期的释放速度比0.25%(w/w)的水凝胶稍快。0.25%(w/w)的Cur β-CD-HA-QSG水凝胶的释放速度相对较慢,尤其是在溶解初期。然而,这三种制剂在24小时内均达到约98%的释放量。0.75%(w/w)和0.50%(w/w)姜黄素含量的HA-QSG水凝胶具有快速的初始释放,可能适合需要快速获得药物的关节内应用,而0.25%(w/w)姜黄素含量的HA-QSG水凝胶具有较慢的释放,可能更有利于持续的关节内给药。图形抽象
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来源期刊
AAPS PharmSciTech
AAPS PharmSciTech 医学-药学
CiteScore
6.80
自引率
3.00%
发文量
264
审稿时长
2.4 months
期刊介绍: AAPS PharmSciTech is a peer-reviewed, online-only journal committed to serving those pharmaceutical scientists and engineers interested in the research, development, and evaluation of pharmaceutical dosage forms and delivery systems, including drugs derived from biotechnology and the manufacturing science pertaining to the commercialization of such dosage forms. Because of its electronic nature, AAPS PharmSciTech aspires to utilize evolving electronic technology to enable faster and diverse mechanisms of information delivery to its readership. Submission of uninvited expert reviews and research articles are welcomed.
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