Mitigating effects of Methyl Jasmonate on photosynthetic inhibition and oxidative stress of pepper (Capsicum annuum L) seedlings under low temperature combined with low light

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-03-28 DOI:10.1016/j.plaphy.2025.109843

Kaiguo Pu, Nenghui Li, Yanqiang Gao, Tiantian Wang, Miao Zhang, Wenli Sun, Jing Li, Jianming Xie

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引用次数: 0

Abstract

Low temperature combined with low light (LL) is a critical abiotic stress that restricting plant growth and yield of pepper (Capsicum annuum L.). Methyl jasmonate (MeJA) is considered with potential benefits for improving plant stress resistance; however, the physiological mechanisms underlying the adaptation of pepper to LL stress have not been explored. This study aimed to investigate the potential mitigating effects of foliar MeJA (200 μmol L⁻¹) application on pepper seedlings subjected to LL stress (10/5 °C, 100 μmol m⁻² s⁻¹) for 168 h. Our results indicated that the application of exogenous MeJA reduced the negative effect on growth inhibition of pepper seedlings caused by LL stress, significantly increased chlorophyll contents and photosynthetic capacity as a result of improved photosynthesis rate. In addition, MeJA reduced the accumulation of reactive oxygen species and malondialdehyde contents induced by LL stress, while enhancing the activities of superoxide dismutase, peroxidase, catalase, ascorbate peroxidase, dehydroascorbate reductase, and monodehydroascorbate reductase as a result of upregulated expression levels of antioxidant enzyme genes (CaSOD, CaPOD, CaCAT, CaAPX, CaGR, CaDHAR, and CaMDHAR). Additionally, it increased the ascorbic acid and reduced glutathione content, while reducing oxidized glutathione content, thereby preventing membrane lipid peroxidation and protecting plants from oxidative damage under LL stress. Furthermore, seedlings treated with MeJA exhibited significantly enhanced soluble sugar and soluble protein contents in leaves. Taken together, present findings indicate that MeJA application may serve as an effective strategy for mitigating LL-induced oxidative stress by maintaining plant growth, enhancing chlorophyll fluorescence, upregulating the antioxidant defence system, optimizing ascorbate-glutathione cycle, and osmotic adjustment.

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茉莉酸甲酯对低温弱光条件下辣椒幼苗光合抑制和氧化应激的缓解作用

低温弱光胁迫是制约辣椒植株生长和产量的重要非生物胁迫。茉莉酸甲酯（MeJA）被认为具有提高植物抗逆性的潜在益处；然而，辣椒对LL胁迫适应的生理机制尚未深入研究。本研究旨在探讨叶面施用MeJA （200 μmol L−1）对LL胁迫（10/5°C, 100 μmol m−2 s−1）168 h辣椒幼苗的缓解作用。结果表明，外源MeJA的施用降低了LL胁迫对辣椒幼苗生长抑制的负面影响，显著提高了叶绿素含量和光合能力，提高了光合速率。此外，MeJA降低了LL胁迫诱导的活性氧积累和丙二醛含量，同时上调了抗氧化酶基因（CaSOD、CaPOD、CaCAT、CaAPX、CaGR、CaDHAR和CaMDHAR）的表达水平，提高了超氧化物歧化酶、过氧化物酶、过氧化氢酶、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶和单脱氢抗坏血酸还原酶的活性。增加抗坏血酸和谷胱甘肽含量，同时降低氧化谷胱甘肽含量，从而防止膜脂过氧化，保护植物免受LL胁迫下的氧化损伤。此外，MeJA处理的幼苗叶片中可溶性糖和可溶性蛋白含量显著提高。综上所述，目前的研究结果表明，MeJA的应用可能通过维持植物生长、增强叶绿素荧光、上调抗氧化防御系统、优化抗坏血酸-谷胱甘肽循环和渗透调节来缓解ll诱导的氧化应激。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.