Sulfonic acid-functionalized covalent organic framework@Ti3C2Tx as efficient solid-phase microextraction blade coating for the extraction of monoamine neurotransmitters in rat serum samples

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2025-03-28 DOI:10.1016/j.chroma.2025.465919

Cheng Yang , Nian Yang , Di Zhao , Zhiyu Zhang , Junying Song , Zhenqiang Zhang , Kai Hu , Shusheng Zhang

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引用次数: 0

Abstract

Herein, a sulfonic-functionalized covalent organic framework@Ti₃C₂T_x nanocomposite (SO₃HCOF@Ti₃C₂T_x) was synthesized and employed as solid phase microextraction (SPME) coating for isolation and extraction of monoamine neurotransmitters (MNTs) from rat serum samples. The resultant composite can combine the characteristics of hydrophilic Ti₃C₂T_x and SO₃HCOF, which endow it has multiple adsorption sites and can provide multiple interactions such as cation exchange, hydrogen bonding and π-π with the target MNTs. The synthesized SO₃HCOF@Ti₃C₂T_x SPME blades have excellent protein exclusion capability, ensuring high adsorption efficiency for MNTs. Under the optimized conditions, the proposed SO₃HCOF@Ti₃C₂T_x blades-based SPME-HPLC method exhibited good linearities (r²≥0.9963), low limits of detection (0.015–0.030 ng mL^-1) and low matrix effect (0.83 %-17.36 %). The recoveries of MNTs in the rat serum were in range of 90.3 %-118.3 %, with RSDs <10.8 %. The SPME-HPLC method was successfully applied for the analysis of 4 MNTs in the serum of depression model rats. This work not only details the development of a multi-functional composite, but it also presents an effective strategy for the determination of trace MNTs in serum sample.

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磺酸功能化共价有机framework@Ti3C2Tx作为高效固相微萃取叶片涂层提取大鼠血清中单胺类神经递质

本文合成了一种磺化共价有机framework@Ti3C2Tx纳米复合材料（SO3HCOF@Ti3C2Tx），并将其用作固相微萃取（SPME）涂层，用于分离和提取大鼠血清样品中的单胺类神经递质（MNTs）。该复合材料结合了Ti3C2Tx和SO3HCOF亲水性的特点，具有多个吸附位点，并能与目标MNTs进行阳离子交换、氢键和π-π等多种相互作用。合成的SO3HCOF@Ti3C2Tx SPME叶片具有优异的蛋白质排除能力，保证了MNTs的高吸附效率。在优化条件下，SO3HCOF@Ti3C2Tx叶片型SPME-HPLC法线性良好（r2≥0.9963），检出限低（0.015 ~ 0.030 ng mL-1），基质效应低（0.83% ~ 17.36%）。mnt在大鼠血清中的加样回收率为90.3% ~ 118.3%，rsd为10.8%。采用SPME-HPLC法对抑郁症模型大鼠血清中4种mnt进行了分析。这项工作不仅详细介绍了多功能复合材料的开发，而且还提出了一种测定血清样品中痕量mnt的有效策略。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.