Development of a highly sensitive field-amplified capillary electrophoresis tandem triple quadrupole mass spectrometry for the quantification of trace methamphetamine in sewage

IF 3.8 2区 化学 Q1 BIOCHEMICAL RESEARCH METHODS
Yanping Wei , Yujie Zhang , Yueting Hu , Fan Shui , Jia Tang , Yiyang Yu , Guocan Zheng , Jing Zeng
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引用次数: 0

Abstract

Accurate quantification of trace levels of illicit drugs in wastewater, such as methamphetamine (MAMP), is crucial for sewage epidemiology. In this study, we developed a novel method that integrates field-amplified sample injection capillary electrophoresis with triple quadrupole mass spectrometry (FASI-CE-QQQ MS). This method enables simultaneous on-line preconcentration and separation, coupled with highly sensitive detection. Validation demonstrated excellent linearity within the range of 1 to 200 ng/L (R² = 0.9996), a limit of detection (LOD) of 0.1 ng/L (S/N = 12.3), high accuracy (RE < 6.4 %) and precision (RSD < 11.4 %). It was successfully applied to quantify MAMP in two unknown water samples, yielding average concentrations of 3.70 ng/L and 84.73 ng/L, respectively. These results were consistent with those obtained from an interlaboratory comparison exercise across China using on-line solid-phase extraction coupled with ultra-high performance liquid chromatography tandem triple quadrupole mass spectrometry (on-line SPE-UHPLC-QQQ MS). Finally, a comprehensive comparison between the newly developed FASI-CE-QQQ MS and the on-line SPE-UHPLC-QQQ MS methods revealed that the FASI-CE-QQQ MS offers significant advantages in terms of sensitivity, rapidity, and reduced solvent and sample consumption, making it a promising approach for the accurate determination of trace drugs in water.
高灵敏度场放大毛细管电泳串联三重四极杆质谱法测定污水中痕量甲基苯丙胺的建立
准确量化废水中痕量非法药物,如甲基苯丙胺(MAMP),对污水流行病学至关重要。在这项研究中,我们开发了一种将场放大样品注射毛细管电泳与三重四极杆质谱(FASI-CE-QQQ MS)相结合的新方法。该方法可同时在线富集和分离,并具有高灵敏度的检测。结果表明,该方法在1 ~ 200 ng/L范围内线性良好(R²= 0.9996),检出限(LOD)为0.1 ng/L (S/N = 12.3),准确度高(RE <;6.4%)和精密度(RSD <;11.4%)。它成功地应用于定量两个未知水样中的MAMP,平均浓度分别为3.70 ng/L和84.73 ng/L。这些结果与在中国使用在线固相萃取-超高效液相色谱串联三重四极杆质谱(在线SPE-UHPLC-QQQ MS)进行的实验室间比较一致。最后,将新建立的FASI-CE-QQQ质谱法与在线SPE-UHPLC-QQQ质谱法进行了综合比较,结果表明FASI-CE-QQQ质谱法在灵敏度、快速、减少溶剂和样品消耗等方面具有显著优势,有望用于水中痕量药物的准确测定。
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来源期刊
Journal of Chromatography A
Journal of Chromatography A 化学-分析化学
CiteScore
7.90
自引率
14.60%
发文量
742
审稿时长
45 days
期刊介绍: The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.
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