Transcriptomic and single-cell transcriptomic sequencing analysis of prevention of Dendrobium officinale flower extract on chronic obstructive pulmonary disease in mice induced by cigarette smoke

IF 3.8 2区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Journal of Functional Foods Pub Date : 2025-04-01 DOI:10.1016/j.jff.2025.106785

Zheng-Wei Liang , Hui Luo , Yun-Jun Zhang , Nian-Guo Bo , Teng Wang , Gen Sha , Yan-Hui Guan , Jia-Kun Fan , Yan Ma , Sheng-Chao Yang , Jun-Wen Chen , Tie-Jun Ling , Ming Zhao

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Abstract

Dendrobium officinale flowers possess anti-aging and nourishing properties for the skin; however, there is a notable deficiency in pharmacological research concerning chronic obstructive pulmonary disease (COPD). To investigate the effects of Dendrobium officinale flower extract (DOFE) on the amelioration of COPD, 29 flavonoids present in DOFE were identified and quantified, which demonstrate antioxidant and anti-inflammatory properties. Pathological observation, RNA-Seq and ScRNA-seq analysis revealed that DOFE treatment decreased the up-expression of genes caused by cigarette smoke (CS), which includes receptors (TLR, TNFR, and NOD), transcription factors (AP-1, STAT1), and key proteins (HSP90, A20, MKP) that contribute to the generation of reactive oxygen species and activation of the NF-κB and NLRP inflammasomes. This resulted in a decrease in secretion of IL-1β, IL-5, MIP-1α, and MIP-1β in neutrophils and macrophages, suppressed the increase in alveolar macrophages. Together, DOFE alleviated pulmonary inflammation, restored antioxidant capacity, and prevented COPD induced by CS.

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铁皮石斛花提取物预防吸烟所致小鼠慢性阻塞性肺疾病的转录组学和单细胞转录组测序分析

铁皮石斛花具有抗衰老和滋养皮肤的特性；然而，在慢性阻塞性肺疾病（COPD）的药理研究方面存在明显不足。为了研究铁皮石斛花提取物（DOFE）对慢性阻塞性肺疾病（COPD）的改善作用，我们对DOFE中29种黄酮类化合物进行了鉴定和定量，这些黄酮类化合物具有抗氧化和抗炎作用。病理观察、RNA-Seq和ScRNA-seq分析显示，DOFE治疗降低了香烟烟雾引起的基因（CS）的上调表达，包括受体（TLR、TNFR和NOD）、转录因子（AP-1、STAT1）和促进活性氧生成和NF-κB和NLRP炎症小体活化的关键蛋白（HSP90、A20、MKP）。导致嗜中性粒细胞和巨噬细胞IL-1β、IL-5、MIP-1α和MIP-1β的分泌减少，抑制肺泡巨噬细胞的增加。总之，DOFE减轻了肺部炎症，恢复了抗氧化能力，预防了CS诱导的COPD。

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来源期刊

Journal of Functional Foods FOOD SCIENCE & TECHNOLOGY-

CiteScore

9.60

自引率

1.80%

发文量

428

审稿时长

76 days

期刊介绍： Journal of Functional Foods continues with the same aims and scope, editorial team, submission system and rigorous peer review. We give authors the possibility to publish their top-quality papers in a well-established leading journal in the food and nutrition fields. The Journal will keep its rigorous criteria to screen high impact research addressing relevant scientific topics and performed by sound methodologies. The Journal of Functional Foods aims to bring together the results of fundamental and applied research into healthy foods and biologically active food ingredients. The Journal is centered in the specific area at the boundaries among food technology, nutrition and health welcoming papers having a good interdisciplinary approach. The Journal will cover the fields of plant bioactives; dietary fibre, probiotics; functional lipids; bioactive peptides; vitamins, minerals and botanicals and other dietary supplements. Nutritional and technological aspects related to the development of functional foods and beverages are of core interest to the journal. Experimental works dealing with food digestion, bioavailability of food bioactives and on the mechanisms by which foods and their components are able to modulate physiological parameters connected with disease prevention are of particular interest as well as those dealing with personalized nutrition and nutritional needs in pathological subjects.