Effects of dietary inclusion of kelp residue on growth performance, blood biochemistry, hepatic metabolism, digestive enzymes and intestinal health of turbot Scophthalmus maximus

IF 2.5 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Feed Science and Technology Pub Date : 2025-03-22 DOI:10.1016/j.anifeedsci.2025.116300

Peiyu Li , Zhidong Song

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引用次数: 0

Abstract

This study investigated the effects of dietary kelp residue (KR) inclusion on growth performance, nutrient utilization, and physiological responses in juvenile turbot (Scophthalmus maximus; initial body weight 35.17 ± 0.25 g) through an 8-week feeding trial. Six isonitrogenous and isolipidic diets containing 0, 3, 6, 9, 12, and 15 % KR were randomly assigned to triplicate tanks. Results revealed growth metrics (weight gain, specific growth rate) and nutrient digestibility coefficients (dry matter, crude lipid, energy) significantly decreased with KR inclusion > 9 %, accompanied by elevated feed conversion ratio compared with the control group (P < 0.05). Protein digestibility declined markedly at KR levels > 12 % (P < 0.05). Serum lipid profiles (triglycerides, low-density lipoprotein) and intestinal tight junction proteins (zona, occludens-1) decreased, while hepatic carnitine palmitoyltransferase-1 activity increased significantly with KR inclusion > 6 % compared with the control group (P < 0.05). KR inclusion > 9 % suppressed hepatic metabolism (alanine aminotransferase, hexokinase) and intestinal antioxidant capacity (superoxide dismutase), concurrently reducing serum albumin and glucose levels. High KR inclusion (12–15 %) induced metabolic dysregulation, evidenced by elevated serum aminotransferases and upregulated intestinal pro-inflammatory cytokines (IL-1β, TNF-α), coupled with diminished metabolic and antioxidant enzyme activities as compared with the control group (P < 0.05). Through quadratic regression analysis, the optimal KR inclusion level was determined as 6.57–6.72 % for maximal growth performance. These findings demonstrate that excessive KR inclusion (>9 %) compromises nutrient assimilation and induces metabolic stress in juvenile turbot, providing critical thresholds for sustainable aquafeed formulation.

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饲料中添加海带渣对大菱鲆生长性能、血液生化、肝脏代谢、消化酶和肠道健康的影响

本试验研究了饲料中添加海带渣（KR）对大菱鲆（Scophthalmus maximus）幼鱼生长性能、养分利用和生理反应的影响；初始体重35.17 ± 0.25 g)，饲喂8周。6种含0、3、6、9、12和15 % KR的等氮等脂饲料被随机分配到3个重复的槽中。结果表明：与对照组相比，添加KR组的生长指标（增重、特定生长率）和营养物质消化系数（干物质、粗脂肪、能量）显著降低（P <； 0.05），饲料系数显著提高（P <； 0.05）。KR水平显著降低蛋白质消化率>； 12 % （P <； 0.05）。血清血脂（甘油三酯，低密度脂蛋白）和肠道紧密连接蛋白（zona, occluden -1）降低，而与对照组相比，含有KR的肝肉碱棕榈酰基转移酶-1活性显著升高>； 6 % （P <； 0.05）。KR内含物>； 9 %抑制肝脏代谢（丙氨酸转氨酶、己糖激酶）和肠道抗氧化能力（超氧化物歧化酶），同时降低血清白蛋白和葡萄糖水平。与对照组相比，高KR包合（12-15 %）诱导代谢失调，表现为血清转氨酶升高，肠道促炎细胞因子（IL-1β, TNF-α）上调，代谢和抗氧化酶活性降低（P <； 0.05）。通过二次回归分析，确定最佳的KR添加水平为6.57 ~ 6.72 %，可获得最大的生长性能。这些研究结果表明，过量的KR （>9 %）会损害营养物质的同化，并引起幼鱼的代谢应激，为可持续的水产饲料配方提供了临界阈值。

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来源期刊

Animal Feed Science and Technology 农林科学-奶制品与动物科学

CiteScore

6.00

自引率

6.20%

发文量

266

审稿时长

3 months

期刊介绍： Animal Feed Science and Technology is a unique journal publishing scientific papers of international interest focusing on animal feeds and their feeding. Papers describing research on feed for ruminants and non-ruminants, including poultry, horses, companion animals and aquatic animals, are welcome. The journal covers the following areas: Nutritive value of feeds (e.g., assessment, improvement) Methods of conserving and processing feeds that affect their nutritional value Agronomic and climatic factors influencing the nutritive value of feeds Utilization of feeds and the improvement of such Metabolic, production, reproduction and health responses, as well as potential environmental impacts, of diet inputs and feed technologies (e.g., feeds, feed additives, feed components, mycotoxins) Mathematical models relating directly to animal-feed interactions Analytical and experimental methods for feed evaluation Environmental impacts of feed technologies in animal production.