Exploring glutathione-decorated micelles for drug delivery: A promise for enhanced cellular uptake

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-28 DOI:10.1016/j.colsurfb.2025.114664

Martyna Truszkowska , Elsa Reisenberger , Gergely Kali , Daniel Stengel , Ahmad Saleh , Anna Seybold , Tobias Kipura , Marcel Kwiatkowski , Andreas Bernkop-Schnürch

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引用次数: 0

Abstract

This study aimed to evaluate the effect of thiolated micelles on the cellular uptake of their payload.

Reduced and oxidized glutathione were covalently attached to palmitic acid via amide bond formation. Micelles formed with these thiolated surfactants (M_P-GSH and M_P-GSSG-P) were evaluated regarding critical micellar concentration (CMC) and hemolytic activity. Cytotoxicity was evaluated on HEK 293 and HeLa cells. Diffusion of micelles in these cells was evaluated by fluorescence correlation spectroscopy (FCS). Furthermore, cellular uptake of micelles containing coumarin-6 as model drug was analyzed by flow cytometry and confocal laser scanning microscopy.

CMC of M_P-GSSG-P, M_P-GSH, and palmitic acid micelles (M_PA) was determined to be 0.455 mM, 0.166 mM, and 0.046 mM, respectively. At a concentration of 0.5 % M_P-GSSG-P, M_P-GSH, and M_PA caused around 80 % hemolysis. In HEK cells, M_P-GSSG-P exhibited toxicity at a concentration of 0.25 %, while M_P-GSH showed toxicity at 0.06 % after 4 hours of incubation. In contrast, HeLa cells were more resilient, with only M_P-GSH showing toxicity at 0.25 %. Diffusivity of M_P-GSH and M_P-GSSG-P within the cells was higher than that of M_PA. Cellular uptake studies demonstrated a significantly (p < 0.05) enhanced internalization of M_P-GSH and M_P-GSSG-P, that was 112-fold and 270-fold higher in HEK 293 cells and 12-fold and 60-fold higher in HeLa cells when compared to M_PA.

These findings suggest that M_P-GSH and M_P-GSSG-P could serve as promising vehicles for enhancing cellular uptake of drugs.

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.