Dynamic whole genome amplification using digital microfluidics for single-cell sequencing

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-03-31 DOI:10.1016/j.cej.2025.162099

Qingyu Ruan, Changrui Shi, Xiaoye Lin, Weidong Ruan, Minghui Xu, Chaoyong Yang, Yukun Ren

{"title":"Dynamic whole genome amplification using digital microfluidics for single-cell sequencing","authors":"Qingyu Ruan, Changrui Shi, Xiaoye Lin, Weidong Ruan, Minghui Xu, Chaoyong Yang, Yukun Ren","doi":"10.1016/j.cej.2025.162099","DOIUrl":null,"url":null,"abstract":"Single-cell whole-genome sequencing (WGS) is primarily utilized to investigate the investigation of genetic alterations between individual cells, particularly focusing on copy number variations. Prior to single-cell WGS, genome amplification of single cells is indispensable, but poses challenges due to the limited amount of DNA and nonuniform amplification. Here, this work presented a digital microfluidic platform to execute automated single-cell WGA based on multiple annealing and looping-based amplification cycles (digital MALBAC). This platform enables us to perform one-step MALBAC by dynamic droplet shuttling between cold plate and heat plate, implementing the entire procedure from single-cell isolation to WGA. Our study demonstrated that digital MALBAC had low genomic amplification bias and high reproducibility, enabling accurate identification of copy number variations (CNVs). Leveraging digital microfluidics with precise temperature control, digital MALBAC offers a user-friendly, rapid, and highly reproducible solution that integrates seamlessly into workflows, positioning it as a promising strategy for biological applications.","PeriodicalId":270,"journal":{"name":"Chemical Engineering Journal","volume":"30 1","pages":""},"PeriodicalIF":13.3000,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical Engineering Journal","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1016/j.cej.2025.162099","RegionNum":1,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, CHEMICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Single-cell whole-genome sequencing (WGS) is primarily utilized to investigate the investigation of genetic alterations between individual cells, particularly focusing on copy number variations. Prior to single-cell WGS, genome amplification of single cells is indispensable, but poses challenges due to the limited amount of DNA and nonuniform amplification. Here, this work presented a digital microfluidic platform to execute automated single-cell WGA based on multiple annealing and looping-based amplification cycles (digital MALBAC). This platform enables us to perform one-step MALBAC by dynamic droplet shuttling between cold plate and heat plate, implementing the entire procedure from single-cell isolation to WGA. Our study demonstrated that digital MALBAC had low genomic amplification bias and high reproducibility, enabling accurate identification of copy number variations (CNVs). Leveraging digital microfluidics with precise temperature control, digital MALBAC offers a user-friendly, rapid, and highly reproducible solution that integrates seamlessly into workflows, positioning it as a promising strategy for biological applications.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.