Fungal chromatin remodeler Isw1 modulates translation via regulating tRNA transcription

IF 16.6 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Nucleic Acids Research Pub Date : 2025-04-01 DOI:10.1093/nar/gkaf225

Jing Wang, Yueqi Zhang, Jingrui Wang, Liuqin Wang, Binshan Li, Shuang Liu, Xuepeng Sun, Zhonghua Ma

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引用次数: 0

Abstract

Chromatin dynamics are essential for regulating DNA processes in response to environmental stimuli. Although ISWI-family enzymes are known to remodel chromatin by sliding nucleosomes in budding yeast, their functional roles and outputs in eukaryotes remain largely unknown. In this study, we investigated chromatin accessibility in the phytopathogenic fungus Fusarium graminearum treated with and without putrescine, a compound that rapidly induces the biosynthesis of the mycotoxin deoxynivalenol (DON). Putrescine globally alters chromatin accessibility, with the ATP-dependent chromatin remodeler FgIsw1 emerging as a key regulator. Unexpectedly, deletion of FgIsw1 did not affect the transcription of DON biosynthesis genes (Tri) but significantly disrupted transfer RNA (tRNA) transcription, leading to a dramatic decline in translation of DON biosynthesis enzymes. Mechanistically, FgIsw1 maintains nucleosome phasing in tRNA chromatin regions, ensuring efficient tRNA transcription. As a result, ΔFgIsw1 was unable to produce DON and lost its virulence on the host plant. These results highlight a novel role of chromatin remodelers in regulating protein translation through the control of tRNA transcription.

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来源期刊

Nucleic Acids Research 生物-生化与分子生物学

CiteScore

27.10

自引率

4.70%

发文量

1057

审稿时长

2 months

期刊介绍： Nucleic Acids Research (NAR) is a scientific journal that publishes research on various aspects of nucleic acids and proteins involved in nucleic acid metabolism and interactions. It covers areas such as chemistry and synthetic biology, computational biology, gene regulation, chromatin and epigenetics, genome integrity, repair and replication, genomics, molecular biology, nucleic acid enzymes, RNA, and structural biology. The journal also includes a Survey and Summary section for brief reviews. Additionally, each year, the first issue is dedicated to biological databases, and an issue in July focuses on web-based software resources for the biological community. Nucleic Acids Research is indexed by several services including Abstracts on Hygiene and Communicable Diseases, Animal Breeding Abstracts, Agricultural Engineering Abstracts, Agbiotech News and Information, BIOSIS Previews, CAB Abstracts, and EMBASE.