Effects of storage time and temperature on coagulation factor and natural anticoagulant activities in healthy individuals.

IF 3.9 2区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Scientific Reports Pub Date : 2025-03-28 DOI:10.1038/s41598-025-95389-w

Ryosuke Nakanishi, Tasuku Sakayori, Daichi Matsui, Mai Kono, Minase Maki, Claire Dunois, Yutaka Komiyama, Hiromi Morisaki

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Abstract

Coagulation test results are influenced by many pre-analytical variables, including clinical sample storage conditions. This study investigated the effects of storage time and temperature on coagulation factor (FII, FV, FVII, FX, FVIII, FIX, FXI, FXII, and FXIII) and natural anticoagulant (antithrombin, protein C, and free protein S) activities. Blood samples were collected from 78 healthy individuals, and 3 samples were used for each coagulation factor and natural anticoagulant. Plasma was tested after centrifugation and storage at room temperature (18 to 25 °C) or refrigerated (2 to 8 °C) for 2, 3, 4, and 5 h, or frozen (- 15 to - 25 °C) for 1, 2, 3, and 4 months. For all storage conditions, the mean percentage change from baseline in evaluated activities was < 15%, except for FV and FVIII. For FV activities after frozen storage at - 15 to - 25 °C and FVIII activities at all storage conditions, the mean percentage change was > 15%. For FV and FVIII activities, the mean percentage change was < 15% after frozen storage at - 75 to - 85 °C for 1, 2, 3, and 4 months. To ensure appropriate clinical testing results, especially for FV and FVIII activity, storage condition and time should be critically controlled.

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贮存时间和温度对健康人凝血因子和天然抗凝血活性的影响。

凝血试验结果受许多分析前变量的影响，包括临床样品储存条件。本研究考察了贮藏时间和温度对凝血因子（FII、FV、FVII、FX、FVIII、FIX、FXI、FXII和FXIII）和天然抗凝血剂（抗凝血酶、蛋白C和游离蛋白S）活性的影响。采集78例健康人的血液，每种凝血因子和天然抗凝剂各取3份。血浆在室温（18 ~ 25℃）或冷藏（2 ~ 8℃）2、3、4、5小时或冷冻（- 15 ~ - 25℃）1、2、3、4个月后进行检测。在所有储存条件下，评估活动中与基线相比的平均百分比变化为15%。FV和FVIII活性的平均百分比变化为

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来源期刊

Scientific Reports Natural Science Disciplines-

CiteScore

7.50

自引率

4.30%

发文量

19567

审稿时长

3.9 months

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