Huy H Pham, Viet Q Tran, Anh H Le, Duy L Nguyen, Toan D Pham, Anh L Vu, Tien K Le, Ho L Le, Bao G Huynh, Tuong M Ho, Lan N Vuong
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引用次数: 0
Abstract
Purpose: This study investigated the impact of low versus high oxygen tension on the oocyte maturation rate in biphasic CAPA-IVM.
Methods: A sibling oocyte pilot study of cumulus-oocyte complexes (COCs) from participants with polycystic ovary syndrome was performed at an academic IVF center in Vietnam from November 2023 to May 2024. At collection, COCs were allocated to undergo CAPA-IVM culture with low oxygen tension (5%) or high oxygen tension (20%). Culture of COCs took place in two benchtop incubators, each equipped with commercial mix-gas bottles to establish the respective oxygen conditions. The primary outcome was the oocyte maturation rate. Secondary outcomes were rates of two-pronuclei fertilization, day-3 embryos, blastocyst formation, and good-quality embryos.
Results: A total of 554 COCs from 20 participants were assigned to the low oxygen (276 COCs) or high oxygen (278 COCs) tension groups. The oocyte maturation rate was significantly lower in the low oxygen tension versus high oxygen tension group (53.6% vs. 65.8%, risk ratio 0.81, 95% confidence interval 0.71-0.94; p=0.004). The two-pronuclei fertilization rate was significantly lower in the low oxygen tension versus high oxygen tension group (61.5% vs. 72.7%, p=0.03). Numbers of day-3 embryos, blastocysts, and good-quality blastocysts were slightly, but not significantly, lower in the low oxygen tension group compared with the high oxygen tension group.
Conclusions: Low oxygen tension of 5% during both steps of biphasic CAPA-IVM reduced the number of matured oocytes and 2-pronuclei fertilized oocytes compared with the use of high oxygen tension (20%) during CAPA-IVM.
期刊介绍：
The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species.
The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.
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