Glycoproteomics of IgA1: uncovering key N-glycan composition in ankylosing spondylitis.

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hui-Ling Chiang, Kuo-Lung Ku, Chien-Hsueh Tung, Kuang-Yung Huang, Ming-Chi Lu, Ning-Sheng Lai
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引用次数: 0

Abstract

Objective: This study aimed to identify distinct IgA1 N-glycan composition in patients with ankylosing spondylitis (AS) compared with healthy controls and to explore their associations with inflammatory markers and disease activity indices.

Methods: Serum samples were collected from 36 patients with AS and 35 healthy controls. The diagnosis of AS was based on the New York criteria. Clinical assessments included inflammatory markers (ESR, CRP, and IgA) and disease activity indices (BASDAI, ASDAS-ESR, and ASDAS-CRP). IgA1 was isolated using affinity purification and gel filtration chromatography, followed by mass spectrometry to identify N-glycans.

Results: Among the 23 detected N-glycan patterns, significant differences were observed in 13 of the 18 N-glycans at the N144 site and in all five N-glycans at the N340 site between patients with AS and controls. Notably, the glycans HexNAc3Hex4NeuAc1, HexNAc4Hex4NeuAc1 and HexNAc5Hex5NeuAc1 at N144 demonstrated strong associations with all three inflammatory markers, including ESR, CRP, and IgA (P < 0.001). Levels of HexNAc4Hex4NeuAc1 were significantly elevated in patients with AS compared with those in the healthy controls. Increased sialylation and galactosylation, along with decreased fucosylation, were noted at N144 of IgA1 in patients with AS. Conversely, no glycans at N340 showed a correlation with all inflammatory markers simultaneously or with any disease activity indicators.

Conclusion: IgA1 from patients with AS exhibited distinct glycosylation traits compared with controls, with elevated levels of HexNAc₄Hex₄NeuAc₁ at N144 associated with inflammatory markers. These findings suggested that differential glycosylation patterns of IgA1 may play a role in the pathogenesis of AS.

IgA1的糖蛋白组学:揭示强直性脊柱炎的关键n -聚糖组成。
目的:本研究旨在鉴定强直性脊柱炎(AS)患者与健康对照者不同的IgA1 n -聚糖组成,并探讨其与炎症标志物和疾病活动性指标的关系。方法:采集36例AS患者和35例健康对照者的血清标本。AS的诊断是基于纽约标准。临床评估包括炎症标志物(ESR、CRP和IgA)和疾病活动性指数(BASDAI、ASDAS-ESR和ASDAS-CRP)。采用亲和纯化和凝胶过滤层析分离IgA1,然后采用质谱法鉴定n -聚糖。结果:在检测到的23种n -聚糖模式中,AS患者与对照组之间在N144位点的18种n -聚糖中有13种存在显著差异,在N340位点的所有5种n -聚糖中有显著差异。值得注意的是,N144位点的糖链HexNAc3Hex4NeuAc1、HexNAc4Hex4NeuAc1和HexNAc5Hex5NeuAc1与所有三种炎症标志物,包括ESR、CRP和IgA有很强的相关性(P结论:与对照组相比,AS患者的IgA1表现出明显的糖基化特征,N144位点的HexNAc₄hex4neuac 1水平升高与炎症标志物相关。这些发现提示IgA1的不同糖基化模式可能在AS的发病机制中发挥作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Glycobiology
Glycobiology 生物-生化与分子生物学
CiteScore
7.50
自引率
4.70%
发文量
73
审稿时长
3 months
期刊介绍: Established as the leading journal in the field, Glycobiology provides a unique forum dedicated to research into the biological functions of glycans, including glycoproteins, glycolipids, proteoglycans and free oligosaccharides, and on proteins that specifically interact with glycans (including lectins, glycosyltransferases, and glycosidases). Glycobiology is essential reading for researchers in biomedicine, basic science, and the biotechnology industries. By providing a single forum, the journal aims to improve communication between glycobiologists working in different disciplines and to increase the overall visibility of the field.
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