Retinoid X receptor γ predicts the prognosis and is associated with immune infiltration in kidney renal clear cell carcinoma: a qRT-PCR, TCGA and in silico research.

Abstract

Background: Kidney clear cell carcinoma (KIRC) stands as one of the most prevalent primary malignant tumors, showcasing significant heterogeneity within the urological system. However, the precise molecular mechanisms underpinning tumorigenesis in KIRC remain elusive. While Retinoid X receptor γ (RXRG) has been implicated in various diseases and human cancers, its specific role in KIRC remains undetermined. This research aimed to investigate the involvement of RXRG in KIRC pathogenesis.

Methods: Quantitative real-time polymerase chain reaction was performed to evaluate the expression levels of RXRG in KIRC. Utilizing RNA-seq data and corresponding clinicopathological information from The Cancer Genome Atlas (TCGA) database, we embarked on an analysis to ascertain the prognostic significance of RXRG in KIRC. Furthermore, bioinformatics analyses were employed to delineate the preliminary molecular mechanisms through which RXRG operates in KIRC tumorigenesis.

Results: Our findings revealed a significant downregulation of RXRG in KIRC tumor tissues compared to normal kidney tissues, as evidenced in local and TCGA cohorts. Diminished RXRG expression correlated with adverse clinicopathological characteristics, including larger tumor size, higher clinical stage, and advanced histologic grade. Cox regression analyses unveiled that reduced RXRG expression was associated with poorer overall survival (OS) and disease-free survival (DFS) rates in KIRC patients. Bioinformatics analyses indicated that the RXRG-related differentially expressed genes (DEGs) were involved in tumorigenesis and metabolism by regulating a series of signaling pathways. Using ssGSEA, we found that RXRG expression was significantly associated with NK cells and macrophages.

Conclusion: Our study provides new insights and evidence that RXRG is involved in the tumorigenesis of KIRC and may be a suitable target for immunotherapy in KIRC.