Selection of Optimal Protocol for Decellularization of Extracellular Matrix of Mesenchymal Stromal Cells of Human Umbilical Cord Tissue.

IF 0.9 4区医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL

Bulletin of Experimental Biology and Medicine Pub Date : 2025-02-01 Epub Date: 2025-03-29 DOI:10.1007/s10517-025-06369-z

E S Kochetova, D K Matveeva, A E Melik-Pashayev, E R Andreeva, Yu A Romanov, L B Buravkova

引用次数: 0

Abstract

To obtain extracellular matrix (ECM) of umbilical cord multipotent mesenchymal stromal cells (UC-MSCs), six decellularization protocols were used, based on physical (freeze/thaw) and chemical (Triton-X100, CHAPS, SDS, and NP-40) methods. The quality of decellularization was assessed using phase-contrast and fluorescence microscopy, quantitative image analysis was performed as well. It was shown that protocols based on CHAPS/NH4OH and Triton X-100/NH4OH are optimal for decellularizing UC-MSC layers and provide high-quality ECM. The SDS-based protocol may be useful when certain requirements to matrix structure are specified. The presented data will be in demand for the development of biomedical products.

查看原文本刊更多论文

人脐带组织间充质间质细胞细胞外基质脱细胞最佳方案的选择。

为了获得脐带多能间充质基质细胞（UC-MSCs）的细胞外基质（ECM），基于物理（冷冻/解冻）和化学（Triton-X100， CHAPS， SDS和NP-40）方法，使用了六种脱细胞方案。采用相衬显微镜和荧光显微镜评估脱细胞质量，并进行定量图像分析。结果表明，基于CHAPS/NH4OH和Triton X-100/NH4OH的协议最适合UC-MSC层脱细胞，并提供高质量的ECM。当指定了对矩阵结构的某些要求时，基于sds的协议可能是有用的。所提供的数据将用于生物医学产品的开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Bulletin of Experimental Biology and Medicine 医学-医学：研究与实验

CiteScore

1.50

自引率

14.30%

发文量

265

审稿时长

2 months

期刊介绍： Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.