Identification and Validation of Genus/Species-Specific Short InDels in Dairy Ruminants.

IF 2.3 2区农林科学 Q1 VETERINARY SCIENCES

BMC Veterinary Research Pub Date : 2025-03-28 DOI:10.1186/s12917-025-04694-z

Gianfranco Cosenza, Andrea Fulgione, Sara Albarella, Francesca Ciotola, Vincenzo Peretti, Daniela Gallo, Alfredo Pauciullo

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引用次数: 0

Abstract

Background: Over the past thirty years, the identification of species-specific molecular markers has significantly advanced our understanding of genetic diversity in both plants and animals. Among these, short InDels have emerged as vital genomic features, contributing more to sequence divergence than single nucleotide polymorphisms do in closely related species. This study aimed to identify specific InDels for Bos taurus, Bubalus bubalis, Capra hircus, and Ovis aries via an in silico approach and validated them in 400 individuals (100 for each species).

Results: We identified and characterized short, specific InDels in the sequences of the CSN1S1, CSN1S2, MSTN, and PRLR genes, which can be used for species identification of Capra hircus, Ovis aries, Bos taurus, and Bubalus bubalis, respectively. We developed a Tetraplex Specific PCR assay to enable efficient discrimination among these species.

Conclusions: This study highlights the utility of InDels as biallelic, codominant markers that are cost-effective and easy to analyse, providing valuable tools for genetic diversity analysis and species identification.

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乳反刍动物属/种特异性短序列的鉴定与验证。

背景：在过去的三十年中，物种特异性分子标记的鉴定大大提高了我们对植物和动物遗传多样性的认识。其中，较短的InDels已成为重要的基因组特征，比单核苷酸多态性在密切相关的物种中对序列差异的贡献更大。本研究旨在通过计算机方法鉴定金牛、山羊、山羊和羊的特异性InDels，并在400个个体（每个物种100个）中进行验证。结果：在CSN1S1、CSN1S2、MSTN和PRLR基因序列中分别鉴定出了短而特异的InDels，可分别用于山羊（Capra hircus）、山羊（Ovis aries）、牛（Bos taurus）和山羊（Bubalus bubalis）的物种鉴定。我们开发了一种四聚体特异性PCR检测，以使这些物种之间的有效区分。结论：该研究突出了InDels作为双等位共显性标记的实用性，该标记具有成本效益和易于分析的特点，为遗传多样性分析和物种鉴定提供了有价值的工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Veterinary Research VETERINARY SCIENCES-

CiteScore

4.80

自引率

3.80%

发文量

420

审稿时长

3-6 weeks

期刊介绍： BMC Veterinary Research is an open access, peer-reviewed journal that considers articles on all aspects of veterinary science and medicine, including the epidemiology, diagnosis, prevention and treatment of medical conditions of domestic, companion, farm and wild animals, as well as the biomedical processes that underlie their health.