Impacts of Biodegradable Microplastics on Rhizosphere Bacterial Communities of Arabidopsis thaliana: Insights into Root Hair-Dependent Colonization.

Abstract

Biodegradable microplastics (MPs) affect plant health by altering rhizosphere microbial communities. Root hairs create a unique niche for diverse microbes, but the effects of biodegradable MPs on root hair-dependent bacterial colonization are unclear, particularly the direct relationship between microbes in the rhizosphere and bulk soil. Here, the effects of polybutylene adipate terephthalate (PBAT) MPs on root hair-dependent bacterial colonization and diversity in the rhizosphere were revealed using an absolute quantitative method and in-situ zymography with two genotypes of Arabidopsis thaliana (long root hair, wild-type, WT and short root hair, rop2-1 mutant, ROP). The results showed that rhizosphere enzyme activity hotspots, bacterial diversity, and colonization increased from ROP to WT plants. PBAT MPs reduced root hair-dependent bacterial colonization and β-glucosidase hotspots by 17.1% and 9.8%, respectively. Despite increasing bacterial absolute abundance in both rhizosphere and bulk soil, PBAT MPs diminished bacterial community modularity and shifted bacterial life strategies from K- to r-strategy via elevated rRNA (rrn) copy numbers and copiotroph/oligotroph ratio. This study indicated that PBAT MPs decreased root hair-dependent bacterial colonization and diversity in the rhizosphere by altering the microbial life history strategies and increasing copiotrophic abundance. This study explained the effects of PBAT MPs on rhizosphere bacterial colonization and diversity from the perspective of root hairs.