Characterization of eosinophil populations in a murine model of type 2 airway inflammation via flow cytometry

Eye & ENT Research Pub Date : 2025-03-30 DOI:10.1002/eer3.70009

Huiqing Wang, Qidi Zhang, Yang Wang, Rongqing Zhang, Bing Yan

{"title":"Characterization of eosinophil populations in a murine model of type 2 airway inflammation via flow cytometry","authors":"Huiqing Wang, Qidi Zhang, Yang Wang, Rongqing Zhang, Bing Yan","doi":"10.1002/eer3.70009","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Purpose</h3>\n \n <p>This study aims to optimize experimental conditions to obtain a larger quantity of eosinophils from the type 2 inflammation mouse model, which will serve as a basis for further investigation into the role and mechanisms of eosinophils in type 2 inflammation.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>We used a mouse model of type 2 inflammation and isolated potential eosinophil-resident tissues (bone marrow, spleen, and nasal mucosa) for flow cytometry analysis. By combining forward scatter, side scatter, and the cell population identified as CD45<sup>+</sup> (protein tyrosine phosphatase receptor type C) CD11b<sup>+</sup> (integrin alpha-M) Ly6G<sup>−</sup> (lymphocyte antigen 6 family member G) Siglec-F<sup>+</sup> (sialic acid-binding immunoglobulin-like lectin F), we characterized eosinophils.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>The results showed that the number and proportion of eosinophils in bone marrow were numerically higher compared to the spleen and nasal mucosa. Further exploration of conditions may provide guarantees for the later development of mechanism research.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>These findings provide clues for further optimizing experimental conditions to obtain sufficient eosinophils from bone marrow for mechanistic studies. They also offer a potential framework for exploring phenotypic heterogeneity and functional studies of eosinophils in various tissues.</p>\n </section>\n </div>","PeriodicalId":100519,"journal":{"name":"Eye & ENT Research","volume":"2 1","pages":"62-69"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/eer3.70009","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Eye & ENT Research","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/eer3.70009","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose

This study aims to optimize experimental conditions to obtain a larger quantity of eosinophils from the type 2 inflammation mouse model, which will serve as a basis for further investigation into the role and mechanisms of eosinophils in type 2 inflammation.

Methods

We used a mouse model of type 2 inflammation and isolated potential eosinophil-resident tissues (bone marrow, spleen, and nasal mucosa) for flow cytometry analysis. By combining forward scatter, side scatter, and the cell population identified as CD45⁺ (protein tyrosine phosphatase receptor type C) CD11b⁺ (integrin alpha-M) Ly6G⁻ (lymphocyte antigen 6 family member G) Siglec-F⁺ (sialic acid-binding immunoglobulin-like lectin F), we characterized eosinophils.

Results

The results showed that the number and proportion of eosinophils in bone marrow were numerically higher compared to the spleen and nasal mucosa. Further exploration of conditions may provide guarantees for the later development of mechanism research.

Conclusion

These findings provide clues for further optimizing experimental conditions to obtain sufficient eosinophils from bone marrow for mechanistic studies. They also offer a potential framework for exploring phenotypic heterogeneity and functional studies of eosinophils in various tissues.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Eye & ENT Research

自引率

0.00%

发文量