Characterization of eosinophil populations in a murine model of type 2 airway inflammation via flow cytometry

Eye & ENT Research Pub Date : 2025-03-30 DOI:10.1002/eer3.70009

Huiqing Wang, Qidi Zhang, Yang Wang, Rongqing Zhang, Bing Yan

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Abstract

Purpose

This study aims to optimize experimental conditions to obtain a larger quantity of eosinophils from the type 2 inflammation mouse model, which will serve as a basis for further investigation into the role and mechanisms of eosinophils in type 2 inflammation.

Methods

We used a mouse model of type 2 inflammation and isolated potential eosinophil-resident tissues (bone marrow, spleen, and nasal mucosa) for flow cytometry analysis. By combining forward scatter, side scatter, and the cell population identified as CD45⁺ (protein tyrosine phosphatase receptor type C) CD11b⁺ (integrin alpha-M) Ly6G⁻ (lymphocyte antigen 6 family member G) Siglec-F⁺ (sialic acid-binding immunoglobulin-like lectin F), we characterized eosinophils.

Results

The results showed that the number and proportion of eosinophils in bone marrow were numerically higher compared to the spleen and nasal mucosa. Further exploration of conditions may provide guarantees for the later development of mechanism research.

Conclusion

These findings provide clues for further optimizing experimental conditions to obtain sufficient eosinophils from bone marrow for mechanistic studies. They also offer a potential framework for exploring phenotypic heterogeneity and functional studies of eosinophils in various tissues.

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通过流式细胞术表征小鼠2型气道炎症模型中的嗜酸性粒细胞群体

本研究旨在优化实验条件，从2型炎症小鼠模型中获得更大量的嗜酸性粒细胞，为进一步研究嗜酸性粒细胞在2型炎症中的作用和机制奠定基础。方法采用小鼠2型炎症模型和分离的潜在嗜酸性粒细胞驻留组织（骨髓、脾脏和鼻黏膜）进行流式细胞术分析。通过结合前向散射、侧向散射和鉴定为CD45+（蛋白酪氨酸磷酸酶受体C型）CD11b+（整合素α - m） Ly6G−（淋巴细胞抗原6家族成员G） siglece -F+（唾液酸结合免疫球蛋白样凝集素F）的细胞群，我们鉴定了嗜酸性粒细胞。结果小鼠骨髓中嗜酸性粒细胞的数量和比例明显高于脾脏和鼻黏膜。对条件的进一步探索可为今后机理研究的开展提供保障。结论本研究结果为进一步优化从骨髓中获得充足嗜酸性粒细胞的实验条件提供了线索。它们也为探索各种组织中嗜酸性粒细胞的表型异质性和功能研究提供了一个潜在的框架。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Eye & ENT Research

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