Metabolomics Based Exploration of the Mechanism of Action of Tripterygium Glycosides in Diabetic Kidney Disease

IF 1.8 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Biomedical Chromatography Pub Date : 2025-03-31 DOI:10.1002/bmc.70071

Li Long, Jianfeng Yu, Jingsong Jin, Jibo Zhang

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引用次数: 0

Abstract

Tripterygium glycosides (TGs), the primary active components of Tripterygium wilfordii, have demonstrated therapeutic efficacy in treating diabetic kidney disease (DKD). However, the precise mechanisms underlying their action remain elusive, limiting the full realization of their medicinal potential. This study employed serum metabolomics based on liquid chromatography–mass spectrometry (LC-MS) analysis to elucidate the mechanisms by which TGs combat DKD. We evaluated the protective effects of TGs on DKD following treatment. Serum samples were collected before and after treatment, and their metabolic profiles were analyzed using LC-MS. Our metabolomics analysis revealed that TGs significantly modulated the hedgehog signaling pathway, a key metabolic pathway implicated in DKD pathogenesis. This study represents the first comprehensive investigation of the metabolic pathways regulated by TGs in the context of DKD using a metabolomics approach. Our findings provide a robust theoretical foundation for the more effective utilization and potential combination therapies involving TGs in the management of DKD. These insights pave the way for further research and development of targeted therapeutic strategies for this challenging condition.

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三尖杉苷（TGs）是三尖杉的主要活性成分，在治疗糖尿病肾病（DKD）方面具有显著疗效。然而，其作用的确切机制仍然难以捉摸，限制了其药用潜力的充分发挥。本研究采用基于液相色谱-质谱联用技术（LC-MS）的血清代谢组学分析方法来阐明 TGs 治疗 DKD 的机制。我们评估了总胆固醇在治疗后对 DKD 的保护作用。我们收集了治疗前后的血清样本，并使用 LC-MS 分析了它们的代谢谱。我们的代谢组学分析表明，TGs 能显著调节刺猬信号通路，这是一种与 DKD 发病机制有关的关键代谢通路。这项研究是首次利用代谢组学方法对DKD背景下受TGs调控的代谢通路进行的全面调查。我们的研究结果为在治疗 DKD 的过程中更有效地利用总胆固醇和潜在的联合疗法提供了坚实的理论基础。这些见解为进一步研究和开发针对这一挑战性疾病的靶向治疗策略铺平了道路。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biomedical Chromatography 生物-分析化学

CiteScore

3.60

自引率

5.60%

发文量

268

审稿时长

2.3 months

期刊介绍： Biomedical Chromatography is devoted to the publication of original papers on the applications of chromatography and allied techniques in the biological and medical sciences. Research papers and review articles cover the methods and techniques relevant to the separation, identification and determination of substances in biochemistry, biotechnology, molecular biology, cell biology, clinical chemistry, pharmacology and related disciplines. These include the analysis of body fluids, cells and tissues, purification of biologically important compounds, pharmaco-kinetics and sequencing methods using HPLC, GC, HPLC-MS, TLC, paper chromatography, affinity chromatography, gel filtration, electrophoresis and related techniques.