A Rapid and Simple UHPLC- DAD Method for Individual Anthocyanin Analysis: Optimization and Validation for Wild Mediterranean Berries and Fruit Samples

IF 2.6 3区农林科学 Q2 FOOD SCIENCE & TECHNOLOGY

Food Analytical Methods Pub Date : 2024-12-04 DOI:10.1007/s12161-024-02726-2

Erika N. Vega, Virginia Fernández-Ruiz, María Cortes Sánchez-Mata, Montaña Cámara, Patricia Morales

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Abstract

Anthocyanins have gained significant attention for their potent colorant capacity and multifaceted biological activities. As a result, the quantification of anthocyanin content in wild berries and the identification of anthocyanin profiles have become increasingly important, as well as the optimization of methods for these determinations. The present study shows a rapid UHPLC-DAD method for identifying and quantifying individual anthocyanins in selected wild berries and fruit samples of Mediterranean origin, namely, Prunus avium, Fragaria vesca, and Vaccinium myrtillus. Moreover, the applicability of the developed method to different food matrices was verified in cultivated berries, specifically, Punica granatum, Fragaria x ananassa, Rubus spp., and Vaccinium corymbosum. The developed method showed great sensitivity (LOD of 23.92 ng/g, LOQ of 79.73 ng/g), precision (RSD below 2.599%), and accuracy (recovery percentages of 97.97 and 98.88%). It successfully quantified individual anthocyanins (delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, petunidin-3-O-glucoside, pelargodinin-3-O-glucoside, malvidin-3-O-glucoside) across different berries and fruit samples. The developed methodology allows to conduct routine analysis without the need for mass spectrometry. The range of total anthocyanins quantified in wild berries and fruit samples was 0.03 to 7.66 mg/g of powdered extract corresponding to Prunus avium L. and Vaccinium corymbosum L., respectively.

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快速简便的UHPLC- DAD法分析地中海野生浆果和水果样品中单个花青素的优化与验证

花青素因其强大的着色剂功能和多方面的生物活性而受到广泛关注。因此，野生浆果中花青素含量的定量测定和花青素谱的鉴定以及测定方法的优化变得越来越重要。本研究建立了一种快速的UHPLC-DAD方法，用于鉴定和定量地中海野生浆果和水果样品中的单个花青素，即Prunus avium， Fragaria vesca和Vaccinium myrtillus。此外，该方法对不同食物基质的适用性在栽培浆果中得到了验证，具体为石榴（Punica granatum）、花椒（Fragaria x ananassa）、红莓（Rubus spp）和玉米苗（vacinium corymbosum）。该方法灵敏度高（定量限23.92 ng/g，定量限79.73 ng/g），精密度高（RSD < 2.599%），准确度高（回收率分别为97.97和98.88%）。它成功地定量了不同浆果和水果样品中的单个花青素（飞鸽苷-3- o -葡萄糖苷，花青素-3- o -葡萄糖苷，花青素-3- o -芦丁糖苷，花青素-3- o -芦丁糖苷，马尾草苷-3- o -葡萄糖苷，马尾草苷-3- o -葡萄糖苷）。开发的方法允许进行常规分析，而不需要质谱分析。野生浆果和水果样品中总花青素的定量范围分别为0.03 ~ 7.66 mg/g，对应于李树（Prunus avium L.）和雪梨（Vaccinium corymbosum L.）粉末提取物。

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来源期刊

Food Analytical Methods 农林科学-食品科技

CiteScore

6.00

自引率

3.40%

发文量

244

审稿时长

3.1 months

期刊介绍： Food Analytical Methods publishes original articles, review articles, and notes on novel and/or state-of-the-art analytical methods or issues to be solved, as well as significant improvements or interesting applications to existing methods. These include analytical technology and methodology for food microbial contaminants, food chemistry and toxicology, food quality, food authenticity and food traceability. The journal covers fundamental and specific aspects of the development, optimization, and practical implementation in routine laboratories, and validation of food analytical methods for the monitoring of food safety and quality.