Construction of a competing endogenous RNA regulatory network in pterygium and role of hsa_circ_0081682 in fibroblast proliferation, migration, and apoptosis

IF 3 2区医学 Q1 OPHTHALMOLOGY

Experimental eye research Pub Date : 2025-03-29 DOI:10.1016/j.exer.2025.110365

Xinyu Tang , Qiaodan Yang , Yulian Dou , Ruiying Zhang , Ming Yan

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引用次数: 0

Abstract

Pterygium is a fibrovascular growth associated with chronic inflammation, tissue remodeling, and angiogenesis, which invades the cornea. Circular RNAs (circRNAs) are emerging as pivotal role in many diseases, but their role in pterygium remains unclear. We performed circRNA and miRNA expression profiling on pterygium and conjunctival tissues, then the circRNA-miRNA-mRNA regulatory network was constructed. Bioinformatics was used to predict downstream pathways. Pterygium fibroblasts were used for experiments assessing proliferation (CCK8, EdU), migration (wound healing, transwell), and apoptosis (AnnexinV-FITC/PI). We identified 162 differentially expressed circRNAs and 96 miRNAs. Key pathways involved in pterygium pathogenesis, including focal adhesion and PI3K-Akt signaling, were predicted. Hsa_circ_0081862 was downregulated in pterygium tissues and fibroblasts, inhibiting fibroblast proliferation and migration while promoting apoptosis. This research constructed a ceRNA network and identified hsa_circ_0081682 as the potential diagnostic marker for pterygium. This research contributes to the understanding of biochemical basis of pterygium, which may facilitate the development of targeted strategies for its management and prevention.

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在翼状胬肉中竞争性内源性RNA调控网络的构建及hsa_circ_0081682在成纤维细胞增殖、迁移和凋亡中的作用

翼状胬肉是一种与慢性炎症、组织重塑和血管生成有关的纤维血管生长，它侵入角膜。环状rna （circRNAs）在许多疾病中发挥着关键作用，但它们在翼状胬肉中的作用尚不清楚。我们对翼状胬肉和结膜组织进行circRNA和miRNA表达谱分析，构建circRNA-miRNA- mrna调控网络。生物信息学用于预测下游通路。利用翼状胬肉成纤维细胞进行增殖（CCK8, EdU）、迁移（伤口愈合，transwell）和凋亡（AnnexinV-FITC/PI）的实验。我们鉴定了162个差异表达的circrna和96个mirna。预测了翼状胬肉发病的关键途径，包括局灶性粘连和PI3K-Akt信号传导。Hsa_circ_0081862在翼状胬肉组织和成纤维细胞中下调，抑制成纤维细胞增殖和迁移，促进细胞凋亡。本研究构建了ceRNA网络，确定hsa_circ_0081682为潜在的翼状胬肉诊断标志物。本研究有助于了解翼状胬肉的生化基础，有助于制定有针对性的治疗和预防策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Experimental eye research 医学-眼科学

CiteScore

6.80

自引率

5.90%

发文量

323

审稿时长

66 days

期刊介绍： The primary goal of Experimental Eye Research is to publish original research papers on all aspects of experimental biology of the eye and ocular tissues that seek to define the mechanisms of normal function and/or disease. Studies of ocular tissues that encompass the disciplines of cell biology, developmental biology, genetics, molecular biology, physiology, biochemistry, biophysics, immunology or microbiology are most welcomed. Manuscripts that are purely clinical or in a surgical area of ophthalmology are not appropriate for submission to Experimental Eye Research and if received will be returned without review.