Transcriptome analysis of the ovary and testis of the pearl oyster Pinctada fucata: Identification of genes and pathways involved in gonadal development

IF 2.2 2区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Animal Reproduction Science Pub Date : 2025-03-27 DOI:10.1016/j.anireprosci.2025.107832

Ying Guo , Pei Wang , Ruolin Zhu , Tangrongjun Guo , Weiwei Chen , Xiuxiu Sang , Lirong Bai , Xianwei Fan , Dahui Yu

{"title":"Transcriptome analysis of the ovary and testis of the pearl oyster Pinctada fucata: Identification of genes and pathways involved in gonadal development","authors":"Ying Guo , Pei Wang , Ruolin Zhu , Tangrongjun Guo , Weiwei Chen , Xiuxiu Sang , Lirong Bai , Xianwei Fan , Dahui Yu","doi":"10.1016/j.anireprosci.2025.107832","DOIUrl":null,"url":null,"abstract":"<div><div><em>Pinctada fucata</em> is a commercially vital species in global pearl aquaculture, producing high-quality pearls. To investigate the molecular regulatory mechanisms of gonadal development in <em>P. fucata</em>, transcriptome analysis was employed to compare expression profiles between testis and ovary across four key developmental stages. A total of 56.86 Gb of clean data was generated, including 392,510 circular consensus sequencing reads, among which 292,295 full-length non-chimeric (FLNC) sequences were identified. A transcript clustering analysis of FLNC reads revealed 89,645 high-quality consensus sequences. 17,646 gene loci were identified, including 8588 novel loci and 28,121 newly discovered transcripts, of which 17,350 were successfully annotated. The boundaries of 12,040 genes on the chromosomes were corrected, and 10,761 complete open reading frame sequences. 281 genes related to gonadal development were identified, including 186 genes with full-length cDNAs in the PacBio library. The study found that <em>HUS1-like</em>, <em>MAD2A-X1</em>, and <em>BLM</em> were stage-specifically upregulated during ovarian maturation, ensuring the accuracy of meiosis. Meanwhile, <em>NR0B1</em>, <em>ETV7L-X5</em>, and <em>CAPRIN1-X2</em> promoted testicular maturation by regulating somatic cell differentiation and the germ cell microenvironment. KEGG enrichment analysis identified key pathways involved in gonadal development, including the ribosome, oxidative phosphorylation, DNA replication, and lysosome. Fatty acid metabolism was linked to ovarian maturation, while the FoxO and ErbB signaling pathways were associated with testicular maturation. These findings offer valuable insights into the molecular mechanisms regulating gonadal development in <em>P. fucata</em> and enhance genomic resources for this economically important species.</div></div>","PeriodicalId":7880,"journal":{"name":"Animal Reproduction Science","volume":"276 ","pages":"Article 107832"},"PeriodicalIF":2.2000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Reproduction Science","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0378432025000715","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}

引用次数: 0

Abstract

Pinctada fucata is a commercially vital species in global pearl aquaculture, producing high-quality pearls. To investigate the molecular regulatory mechanisms of gonadal development in P. fucata, transcriptome analysis was employed to compare expression profiles between testis and ovary across four key developmental stages. A total of 56.86 Gb of clean data was generated, including 392,510 circular consensus sequencing reads, among which 292,295 full-length non-chimeric (FLNC) sequences were identified. A transcript clustering analysis of FLNC reads revealed 89,645 high-quality consensus sequences. 17,646 gene loci were identified, including 8588 novel loci and 28,121 newly discovered transcripts, of which 17,350 were successfully annotated. The boundaries of 12,040 genes on the chromosomes were corrected, and 10,761 complete open reading frame sequences. 281 genes related to gonadal development were identified, including 186 genes with full-length cDNAs in the PacBio library. The study found that HUS1-like, MAD2A-X1, and BLM were stage-specifically upregulated during ovarian maturation, ensuring the accuracy of meiosis. Meanwhile, NR0B1, ETV7L-X5, and CAPRIN1-X2 promoted testicular maturation by regulating somatic cell differentiation and the germ cell microenvironment. KEGG enrichment analysis identified key pathways involved in gonadal development, including the ribosome, oxidative phosphorylation, DNA replication, and lysosome. Fatty acid metabolism was linked to ovarian maturation, while the FoxO and ErbB signaling pathways were associated with testicular maturation. These findings offer valuable insights into the molecular mechanisms regulating gonadal development in P. fucata and enhance genomic resources for this economically important species.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Animal Reproduction Science 农林科学-奶制品与动物科学

CiteScore

4.50

自引率

9.10%

发文量

136

审稿时长

54 days

期刊介绍： Animal Reproduction Science publishes results from studies relating to reproduction and fertility in animals. This includes both fundamental research and applied studies, including management practices that increase our understanding of the biology and manipulation of reproduction. Manuscripts should go into depth in the mechanisms involved in the research reported, rather than a give a mere description of findings. The focus is on animals that are useful to humans including food- and fibre-producing; companion/recreational; captive; and endangered species including zoo animals, but excluding laboratory animals unless the results of the study provide new information that impacts the basic understanding of the biology or manipulation of reproduction. The journal''s scope includes the study of reproductive physiology and endocrinology, reproductive cycles, natural and artificial control of reproduction, preservation and use of gametes and embryos, pregnancy and parturition, infertility and sterility, diagnostic and therapeutic techniques. The Editorial Board of Animal Reproduction Science has decided not to publish papers in which there is an exclusive examination of the in vitro development of oocytes and embryos; however, there will be consideration of papers that include in vitro studies where the source of the oocytes and/or development of the embryos beyond the blastocyst stage is part of the experimental design.