Auxin-induced WRKY23 activates PECTIN LYASE-LIKE1 and PECTIN LYASE-LIKE3 for apoplastic iron reutilization in Arabidopsis roots

IF 6.8 Q1 PLANT SCIENCES

Plant Stress Pub Date : 2025-03-29 DOI:10.1016/j.stress.2025.100830

Yuzhen Zhang , Lihong Hong , Xiaoya Feng , Ru'nan Huang , Li Huang , Yuhuan Wu , Weiwei Chen

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Abstract

The retention and reutilization of apoplastic iron (Fe) are essential for Fe homeostasis in plants, yet the underlying molecular mechanisms remain largely unexplored. Here, we characterized the role of WRKY23, a nucleus-localized transcription factor, in regulating apoplastic Fe retention and reutilization in response to Fe deficiency in Arabidopsis thaliana. Under Fe deficiency, the induction of WRKY23 expression is modulated by local auxin signaling. Once activated, WRKY23 then influenced Fe homeostasis by regulating pectin metabolism and Fe-binding capacity in the cell wall. Notably, WRKY23 could directly bind to W-box motifs in the promoters of target genes, including PECTIN LYASE-LIKE1 (PLL1) and PLL3, activating their transcription. Collectively, our findings support a model in which WRKY23 functions as part of a transcriptional cascade, whereby auxin signaling promotes the role of WRKY23 in regulating pectin degradation and enhancing Fe retention and reutilization in the apoplast, thereby negatively modulating Fe deficiency responses in roots. This research deepens our understanding of plant responses to nutritional stress and may inform strategies for improving crop resilience.

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生长素诱导的WRKY23激活果胶裂解酶- like1和果胶裂解酶- like3，促进拟南芥根系铁的再利用

外体铁（Fe）的保留和再利用对植物体内铁的稳态至关重要，但其潜在的分子机制仍未被充分探索。在这里，我们研究了WRKY23（一种核定位转录因子）在拟南芥（Arabidopsis thaliana）铁缺乏反应中调节外胞铁保留和再利用的作用。缺铁条件下，WRKY23的表达受局部生长素信号的调控。WRKY23一旦被激活，就会通过调节果胶代谢和细胞壁的铁结合能力来影响铁的稳态。值得注意的是，WRKY23可以直接结合靶基因（包括PECTIN LYASE-LIKE1 （PLL1）和PLL3）启动子中的W-box基序，激活它们的转录。总之，我们的研究结果支持WRKY23作为转录级联的一部分的模型，生长素信号促进WRKY23在调节果胶降解和增强铁在外质中的保留和再利用中的作用，从而负调节根的铁缺乏反应。这项研究加深了我们对植物对营养胁迫反应的理解，并可能为提高作物抗逆性提供策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Stress PLANT SCIENCES-

CiteScore

5.20

自引率

8.00%

发文量

审稿时长

63 days

期刊介绍： The journal Plant Stress deals with plant (or other photoautotrophs, such as algae, cyanobacteria and lichens) responses to abiotic and biotic stress factors that can result in limited growth and productivity. Such responses can be analyzed and described at a physiological, biochemical and molecular level. Experimental approaches/technologies aiming to improve growth and productivity with a potential for downstream validation under stress conditions will also be considered. Both fundamental and applied research manuscripts are welcome, provided that clear mechanistic hypotheses are made and descriptive approaches are avoided. In addition, high-quality review articles will also be considered, provided they follow a critical approach and stimulate thought for future research avenues. Plant Stress welcomes high-quality manuscripts related (but not limited) to interactions between plants and: Lack of water (drought) and excess (flooding), Salinity stress, Elevated temperature and/or low temperature (chilling and freezing), Hypoxia and/or anoxia, Mineral nutrient excess and/or deficiency, Heavy metals and/or metalloids, Plant priming (chemical, biological, physiological, nanomaterial, biostimulant) approaches for improved stress protection, Viral, phytoplasma, bacterial and fungal plant-pathogen interactions. The journal welcomes basic and applied research articles, as well as review articles and short communications. All submitted manuscripts will be subject to a thorough peer-reviewing process.