Decoding whole genome of an industrially important thermophilic bacterium Anoxybacteroides rupiense

IF 1 Q4 GENETICS & HEREDITY

Gene Reports Pub Date : 2025-03-26 DOI:10.1016/j.genrep.2025.102207

Uroosa Ejaz , Hassan Ghayas , Sabiha Yousuf , Ayaz Taj , Muhammad Sohail

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引用次数: 0

Abstract

Anoxybacteroides (previously Anoxybacillus) is a relatively new genus, proposed in the year 2000, and comprised of many species which are reported to play a vital role in bioprocessing and bioremediation. Very few reports described the whole genome sequence (WGS) of this genus. Therefore, in this study, WGS of a new strain, UE27, was analyzed. This thermophilic strain was previously isolated from the crocodile pond of Manghopir, Karachi. The genome of A. rupiense (previously Anoxybacillus rupiensis) UE27 was submitted to NCBI GenBank with the accession number JBHLFH010000000.1. Genome size was found to be 3,771,980 bp with 76 contigs and 42.33 % GC content. Among 3789 genes, 3708 were found as protein coding genes. Interestingly, intake prophage genome was found which has not been previously reported in A. rupiense. The prophage region contained 68 coding sequence among which 48 were identified as phage-related genes with 40.09 % GC content. The xylan degradation genes were also annotated in the genome. Moreover, many genes responsible for azo dye degradation, heavy metal detoxification and hydrocarbon removal were found in the genome. Conclusively, this study provided insight into gene repertoire of A. rupiense and a basis for the potential application of this thermophilic bacterium in bioremediation and bioprospecting.

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来源期刊

Gene Reports Biochemistry, Genetics and Molecular Biology-Genetics

CiteScore

3.30

自引率

7.70%

发文量

246

审稿时长

49 days

期刊介绍： Gene Reports publishes papers that focus on the regulation, expression, function and evolution of genes in all biological contexts, including all prokaryotic and eukaryotic organisms, as well as viruses. Gene Reports strives to be a very diverse journal and topics in all fields will be considered for publication. Although not limited to the following, some general topics include: DNA Organization, Replication & Evolution -Focus on genomic DNA (chromosomal organization, comparative genomics, DNA replication, DNA repair, mobile DNA, mitochondrial DNA, chloroplast DNA). Expression & Function - Focus on functional RNAs (microRNAs, tRNAs, rRNAs, mRNA splicing, alternative polyadenylation) Regulation - Focus on processes that mediate gene-read out (epigenetics, chromatin, histone code, transcription, translation, protein degradation). Cell Signaling - Focus on mechanisms that control information flow into the nucleus to control gene expression (kinase and phosphatase pathways controlled by extra-cellular ligands, Wnt, Notch, TGFbeta/BMPs, FGFs, IGFs etc.) Profiling of gene expression and genetic variation - Focus on high throughput approaches (e.g., DeepSeq, ChIP-Seq, Affymetrix microarrays, proteomics) that define gene regulatory circuitry, molecular pathways and protein/protein networks. Genetics - Focus on development in model organisms (e.g., mouse, frog, fruit fly, worm), human genetic variation, population genetics, as well as agricultural and veterinary genetics. Molecular Pathology & Regenerative Medicine - Focus on the deregulation of molecular processes in human diseases and mechanisms supporting regeneration of tissues through pluripotent or multipotent stem cells.