Optimized expression of human interleukin-15 in Nicotiana benthamiana and in vitro assessment of its activity on human keratinocytes

Q1 Immunology and Microbiology

Biotechnology Reports Pub Date : 2025-03-25 DOI:10.1016/j.btre.2025.e00889

Chalatorn Charnsatabut , Pipob Suwanchaikasem , Kaewta Rattanapisit , Iksen Iksen , Varisa Pongrakhananon , Christine Joy I. Bulaon , Waranyoo Phoolcharoen

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引用次数: 0

Abstract

Human interleukin-15 (hIL-15) is a cytokine essential for immune modulation with therapeutic applications in cancer and chronic wound healing. Although hIL-15 is commercially available, large-scale production studies remain limited. With promising clinical trial results, demand for hIL-15 is expected to rise. Plant expression systems offer a sustainable, low-cost alternative for rapid biopharmaceutical production. In this study, we optimized hIL-15 expression in Nicotiana benthamiana and assessed its physicochemical properties and biological activity. We fused hIL-15 to the Fc domain of human IgG1 for efficient purification. Through optimization of the pre- and post-infiltration conditions, we achieved transient expression and recovery at 4 dpi, yielding 33.8 µg/g fresh weight. Peptide mapping confirmed 97 % overall sequence coverage of the primary structure. Treatment with plant-produced hIL-15-Fc effectively promoted human keratinocyte HaCaT cell proliferation and migration in vitro. These findings demonstrated the potential of plant-based platforms for producing therapeutic recombinant hIL-15 that support wound healing.

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人白细胞介素-15在烟叶中的优化表达及其对人角质形成细胞活性的体外评价

人白细胞介素-15 （hIL-15）是一种重要的细胞因子，在癌症和慢性伤口愈合的治疗应用免疫调节。虽然hIL-15已商品化，但大规模生产研究仍然有限。随着有希望的临床试验结果，预计对hIL-15的需求将上升。植物表达系统为快速生物制药生产提供了一种可持续、低成本的替代方案。在本研究中，我们优化了hIL-15在烟叶中的表达，并对其理化性质和生物活性进行了评价。我们将hIL-15融合到人IgG1的Fc结构域以进行高效纯化。通过对浸前和浸后条件的优化，在4 dpi条件下实现了瞬时表达和恢复，产量为33.8µg/g鲜重。肽图谱证实97%的总序列覆盖初级结构。植物源hIL-15-Fc处理能有效促进人角质形成细胞HaCaT细胞的体外增殖和迁移。这些发现证明了基于植物的平台在生产支持伤口愈合的治疗性重组hIL-15方面的潜力。

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.