Two Birds with One Stone: Fe-DNA nanospheres produced via coordination-propelled self-assembly with excellent peroxidase-like property for versatile ratiometric fluorescent assay and cellular imaging

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-03-26 DOI:10.1016/j.bios.2025.117424

Yuwei Zhang , Jiawen Han , Shuai Qin , Juan Wang , Zhihua Lv , Shaojun Dong , Daoqing Fan

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引用次数: 0

Abstract

Exploring novel versatile nanozymes for multi-signal biosensing and cellular application is one of the most promising directions to meet the diversified requirements in this field. Herein, by harnessing coordination-propelled self-assembly between Fe (II) and DNAs, we prepared Fe-DNA nanospheres (Fe-DNA NSs) via a cost-effective one-step hydrothermal method, and pioneered the application of its excellent POD-mimicking property to fluorescent substrates. Initially, we investigated its enzyme-like activity using TMB as canonical colorimetric substrate and screened its catalytic oxidation effects towards different fluorescent substrates, such as T-HCl, AR, OPD and Sc, respectively. Afterwards, by virtue of the contrary fluorescent changes of Sc (decreased FI₄₆₅) and OPD (increased FI₅₆₂) and the cooperative effects of FRET/IFE between them, we devised the first universal Fe-DNA nanospheres-based ratiometric fluorescent (RF) platform. Taking H₂O₂ and glucose as model targets, two RF biosensors based on the alternative direct-nanozyme-catalysis and enzyme/nanozyme-tandem-catalysis were rationally fabricated, respectively. And we further exploited them to evaluate the quality of commercial contact lens care solution, and sensitively determine the blood glucose level of human. Moreover, corresponding cytotoxicity experiments adequately proved the superior biocompatibility of Fe-DNA NSs over most inorganic nanozymes. Furthermore, taking Cy5-labelled A20 strands as templates, we synthesized small-sized (∼60 nm) Fe-DNA fluorescent nanozyme and achieved efficient cellular delivery/imaging. This work not only offered a valid prototype for operating multi-signal-responsive nanozymatic biosensors, but also opened unique avenues for the bio-applications of nucleic acids-originated fluorescent nanozymes in cellular imaging and biotherapy.

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.