Decellularized human amniotic member hydrogel promotes limbal stem cells proliferation

IF 5.4 2区医学 Q1 BIOPHYSICS

Colloids and Surfaces B: Biointerfaces Pub Date : 2025-03-29 DOI:10.1016/j.colsurfb.2025.114656

Yongyao Tan , Wei Wang , Lingjuan Xu , Xiao Zhou , Jiachao Shen , Tianyu Zhou , Chengen Duan , Xuying Wang , Zibin Liu , Mingwu Wang , Guigang Li

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引用次数: 0

Abstract

Allogeneic cultured limbal epithelial stem cell transplantation has shown variable clinical success in treating limbal stem cell deficiency, low success cases are likely due to insufficient stem cell quantity or functional impairment. In this study, we engineered a decellularized amniotic membrane hydrogel (dAM-gel) using a freeze-thaw protocol designed to retain extracellular matrix integrity. Post-processing, collagen content decreased modestly from 313.50 ± 27.89 μg/mg to 284.8 ± 14.82 μg/mg (P = 0.08), while glycosaminoglycan levels shifted from 7.20 ± 1.66 μg/mg to 6.28 ± 0.55 μg/mg (P = 0.27). Crucially, the protocol achieved near-complete DNA removal (7.41 ± 0.78 μg/mg vs. 0.14 ± 0.06 μg/mg) (P < 0.0001), ensuring minimal immunogenicity. Although the dAM-gel demonstrates limited gelation capacity at lower concentrations, it achieves robust gelation at 14 mg/ml, completing the process within 28.26 ± 1.21 minutes. Furthermore, dAM-gel facilitates the migration and proliferation of limbal stem cells, particularly p63 + cells, which are known to correlate with the success of clinical treatments. A plausible explanation for this phenomenon is that dAM-gel contains a high concentration of agrin, which facilitates the proliferation of limbal stem cells while preserving their stemness via the Yap1-cyclin D1 signaling pathway. In conclusion, dAM-gel derived from amniotic membrane presents therapeutic promise for treating limbal stem cell deficiency by enhancing the proliferation of limbal stem cells while maintaining their stem cell phenotype.

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来源期刊

Colloids and Surfaces B: Biointerfaces 生物-材料科学：生物材料

CiteScore

11.10

自引率

3.40%

发文量

730

审稿时长

42 days

期刊介绍： Colloids and Surfaces B: Biointerfaces is an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, having particular relevance to the medical, pharmaceutical, biotechnological, food and cosmetic fields. Submissions that: (1) deal solely with biological phenomena and do not describe the physico-chemical or colloid-chemical background and/or mechanism of the phenomena, and (2) deal solely with colloid/interfacial phenomena and do not have appropriate biological content or relevance, are outside the scope of the journal and will not be considered for publication. The journal publishes regular research papers, reviews, short communications and invited perspective articles, called BioInterface Perspectives. The BioInterface Perspective provide researchers the opportunity to review their own work, as well as provide insight into the work of others that inspired and influenced the author. Regular articles should have a maximum total length of 6,000 words. In addition, a (combined) maximum of 8 normal-sized figures and/or tables is allowed (so for instance 3 tables and 5 figures). For multiple-panel figures each set of two panels equates to one figure. Short communications should not exceed half of the above. It is required to give on the article cover page a short statistical summary of the article listing the total number of words and tables/figures.