Exploring the synergistic potential of Trichoderma gamsii T6085 and Clonostachys rosea IK726 for biological control of Fusarium head blight in wheat

IF 6.9 1区 生物学 Q1 MICROBIOLOGY
Arianna Petrucci , Marco Cesarini , Isabel Vicente , Lucia Merani , Birgit Jensen , David B. Collinge , Sabrina Sarrocco
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Abstract

We explore the combined use of two beneficial fungal isolates, Trichoderma gamsii T6085 (Tg) and Clonostachys rosea IK726 (Cr), to enhance Fusarium head blight (FHB) management by biological control. We found no evidence for mycoparasitism or inhibition via diffusible metabolites, but Tg volatiles inhibited Cr growth slightly. Although Cr reduced Tg spore germination and mycelial growth in liquid culture, this effect seemed absent in planta. The BCAs differently modulated defence-related (DR) genes when colonizing roots or spikes. At seven days post-inoculation (dpi), root-applied Cr, alone and co-inoculated, induced a minor upregulation of PR1. In leaves, a systemic signalling response by root inoculation was detected. In spikes, Pal1, PR1, and Lox1 were upregulated by Cr alone and co-inoculated at 96 hours post-inoculation (hpi). However, Lox1 activation was enhanced by co-inoculation. On spikes inoculated with Fg, the BCAs revealed different patterns of DR gene modulation indicating involvement of different biocontrol mechanisms. In detail, Pgip2 was primarily upregulated at 24 hpi in co-inoculated spikes whereas at 72 hpi activation of DR genes was observed only with Tg. Notably, the disease incidence was reduced by 93 % by co-inoculation. In addition, the inoculum potential of F. graminearum on straw was reduced by all BCAs treatments, with ≥ 96 % reduction of perithecia after six months incubation. Our results show the potential of combining Tg and Cr as a more effective and stable FHB management strategy, than by treatments with the individual strains.
探讨甘氏木霉T6085与玫瑰绿僵菌IK726协同防治小麦赤霉病的潜力
本研究探讨了两种有益真菌甘氏木霉T6085 (Tg)和玫瑰枝孢霉IK726 (Cr)的联合应用,加强对赤霉病(Fusarium head blight, FHB)的生物防治。我们没有发现霉菌寄生或通过弥散代谢物抑制的证据,但Tg挥发物对Cr生长有轻微的抑制作用。虽然Cr在液体培养中降低了Tg孢子的萌发和菌丝的生长,但在植物中似乎没有这种影响。bca在定植根或穗时对防御相关(DR)基因的调节方式不同。在接种后7天(dpi),单独和共接种根施铬诱导PR1轻微上调。在叶片中,通过根接种检测到系统的信号响应。在接种后96 小时(hpi), Cr单独和共接种使Pal1、PR1和Lox1的表达上调。然而,共接种可增强Lox1的激活。在接种Fg的穗上,bca显示出不同的DR基因调节模式,表明参与了不同的生物防治机制。详细地说,Pgip2在共接种的穗状物中主要在24 hpi时上调,而在72 hpi时,DR基因仅与Tg一起激活。值得注意的是,通过共接种,疾病发病率降低了93 %。此外,所有bca处理都降低了禾草镰刀菌在秸秆上的接种势,培养6个月后,周质率降低≥ 96 %。我们的研究结果表明,与单个菌株相比,Tg和Cr联合治疗可能是一种更有效和稳定的FHB管理策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microbiological research
Microbiological research 生物-微生物学
CiteScore
10.90
自引率
6.00%
发文量
249
审稿时长
29 days
期刊介绍: Microbiological Research is devoted to publishing reports on prokaryotic and eukaryotic microorganisms such as yeasts, fungi, bacteria, archaea, and protozoa. Research on interactions between pathogenic microorganisms and their environment or hosts are also covered.
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