A novel fluorescent sensing platform for glutathione based on Förster resonance energy transfer and aggregation-induced emission

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-03-28 DOI:10.1016/j.saa.2025.126131

Mohamed M. El-Wekil , Yousef A. Bin Jardan , Aya M. Mostafa , James Barker , Almontaser Bellah H. Ali

{"title":"A novel fluorescent sensing platform for glutathione based on Förster resonance energy transfer and aggregation-induced emission","authors":"Mohamed M. El-Wekil , Yousef A. Bin Jardan , Aya M. Mostafa , James Barker , Almontaser Bellah H. Ali","doi":"10.1016/j.saa.2025.126131","DOIUrl":null,"url":null,"abstract":"<div><div>Glutathione (GSH) plays essential roles in anti-oxidation and detoxification within the human body. An imbalance in its concentration can lead to serious health conditions. Therefore, accurate monitoring of GSH is critical for maintaining human health. In this study, we present a novel GSH detection method that enhances the fluorescence of α-lipoic acid-functionalized gold nanoclusters (LA@Au NCs) through aggregation induced by zinc and nitrogen co-doped carbon dots (Zn@N-CDs). Additionally, the fluorescence of Zn@N-CDs (donor) decreases upon adding LA@Au NCs (acceptor), indicating Förster resonance energy transfer (FRET) between them. In the presence of GSH, complexation with Zn2+ on the N-CD surface disrupts both the aggregation induced emission (AIE) and FRET mechanisms. This disruption leads to the restoration of N-CD fluorescence while simultaneously quenching the fluorescence of LA@Au NCs. Under optimized conditions, the fluorescence response ratio (F465/F670) is directly proportional to the concentration of GSH within a linear dynamic range of 0.1–90 µM, with a detection limit (S/N = 3) of 0.03 µM. This novel combination paves the way for the development of fluorescent probes for detecting various molecules and biomolecules.</div></div>","PeriodicalId":433,"journal":{"name":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","volume":"337 ","pages":"Article 126131"},"PeriodicalIF":4.3000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1386142525004378","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"SPECTROSCOPY","Score":null,"Total":0}

引用次数: 0

Abstract

Glutathione (GSH) plays essential roles in anti-oxidation and detoxification within the human body. An imbalance in its concentration can lead to serious health conditions. Therefore, accurate monitoring of GSH is critical for maintaining human health. In this study, we present a novel GSH detection method that enhances the fluorescence of α-lipoic acid-functionalized gold nanoclusters (LA@Au NCs) through aggregation induced by zinc and nitrogen co-doped carbon dots (Zn@N-CDs). Additionally, the fluorescence of Zn@N-CDs (donor) decreases upon adding LA@Au NCs (acceptor), indicating Förster resonance energy transfer (FRET) between them. In the presence of GSH, complexation with Zn²⁺ on the N-CD surface disrupts both the aggregation induced emission (AIE) and FRET mechanisms. This disruption leads to the restoration of N-CD fluorescence while simultaneously quenching the fluorescence of LA@Au NCs. Under optimized conditions, the fluorescence response ratio (F₄₆₅/F₆₇₀) is directly proportional to the concentration of GSH within a linear dynamic range of 0.1–90 µM, with a detection limit (S/N = 3) of 0.03 µM. This novel combination paves the way for the development of fluorescent probes for detecting various molecules and biomolecules.

Abstract Image

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.