Effect of cadmium-resistant Proteus mirabilis Ch8 on active ingredients and cadmium content of traditional Chinese medicine Ligusticum sinense cv. Chuanxiong

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2025-03-31 DOI:10.1007/s11104-025-07342-5

Ting Li, Lin Liu, Xiu-wen Bao, Fei-fei Liu, Xuan Zhang, Hao-ran Song, Si-jing Liu, Jing Bai, Qing-he Wang, Fei-lin Yang, Yang Li, Shu-qi Niu, Jin-lin Guo

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引用次数: 0

Abstract

Aims

This study aimed to investigate potential benefits and mechanism of applying Cd-resistant Proteus mirabilis Ch8 to Ligusticum sinense cv. Chuanxiong (Chuanxiong) cultivation. Specifically, we sought to evaluate its effects on plant growth, Cd accumulation, active ingredient content, soil fertility, and rhizosphere microbial community composition.

Methods

A field experiment was conducted with two treatment groups: control group (CK) and Ch8 treatment group. In October, the rhizosphere of Chuanxiong was inoculated with either LB medium (CK) or Ch8 bacterial solution. Plant samples were collected in March of the following year. We measured biomass, Cd content, active ingredient content, rhizosphere soil fertility, and microbial community composition were determined.

Results

Inoculation with Ch8 significantly enhanced the growth of Chuanxiong Rhizomes while decreasing Cd accumulation. Particularly, compared to the CK group, the Ch8 treatment significantly increased the dry weight by 51.99% and decreased the Cd content by 30.62% of Chuanxiong. Additionally, Ch8 effectively inhibited Cd bioaccumulation and translocation in Chuanxiong. Notably, the content of ferulic acid (FA), a key active ingredient, significantly increased by 94.47% in the Ch8 group. Soil analysis revealed that Ch8 application significantly increased the contents of N, P, and K, as well as the residual Cd content in the soil. Furthermore, the structure and function of the rhizosphere microbial community were significantly altered in the Ch8 group. Enrichment of glutathione metabolism and phenylalanine metabolism-related functions was observed, which might contribute to the enhanced accumulation of active ingredients.

Conclusions

Ch8 not only promoted the growth and active ingredient content of Chuanxiong but also reduced Cd accumulation in the plant. These findings provide a scientific basis for utilizing Cd-resistant bacterium to mitigate Cd contamination, improve the quality of medicinal plants, and ensure their sustainable development.

Graphical Abstract

Article Highlights

• Ch8 significantly enhances growth and reduced Cd enrichment in Chuanxiong.

• Inoculation with Ch8 significantly increased active ingredient content in Chuanxiong.

查看原文本刊更多论文

抗镉变形杆菌Ch8对中药川芎有效成分及镉含量的影响Chuanxiong

目的探讨抗cd神奇变形杆菌Ch8对川芎的潜在益处及作用机制。川芎（川芎）栽培。具体来说，我们试图评估其对植物生长、镉积累、有效成分含量、土壤肥力和根际微生物群落组成的影响。方法采用现场试验，分为对照组（CK）和Ch8处理组。10月，将川芎根际分别接种LB培养基（CK）和Ch8菌液。次年3月采集植物样本。我们测量了生物量、Cd含量、有效成分含量、根际土壤肥力和微生物群落组成。结果Ch8可显著促进川芎根茎生长，降低Cd积累。其中，与CK组相比，Ch8处理显著提高了川雄干重51.99%，显著降低了Cd含量30.62%。Ch8能有效抑制川芎体内Cd的积累和转运。其中，关键活性成分阿魏酸（FA）含量在Ch8组显著提高了94.47%。土壤分析表明，施用Ch8显著提高了土壤中N、P、K含量和残留Cd含量。Ch8处理显著改变了根际微生物群落的结构和功能。观察到谷胱甘肽代谢和苯丙氨酸代谢相关功能的富集，这可能有助于增强活性成分的积累。结论sch8不仅能促进川芎的生长和有效成分含量，还能降低植株体内Cd的积累。研究结果为利用耐Cd菌减轻Cd污染、提高药用植物品质、保证药用植物可持续发展提供了科学依据。•Ch8显著促进川雄植株生长，降低Cd富集。•接种Ch8显著提高川芎有效成分含量。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.