Airborne Influenza Virus Surveillance Platform Using Paper-Based Immunosensors and a Growth-Based Virus Aerosol Concentrator

IF 11.3 1区环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL

环境科学与技术 Pub Date : 2025-03-30 DOI:10.1021/acs.est.4c14065

Chanhwi Park, Junbeom Jang, Jaesung Jang

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Abstract

The measurement of respiratory viruses in indoor air is critical for effectively preventing the spread of diseases. This is typically accomplished by counting the nucleic acids or plaques of air-sampled viruses. Herein, we present a growth-based airborne virus surveillance (G-AVS) platform based on paper-based electrochemical immunosensors for targeting hemagglutinin (HA) and nucleoprotein (NP), and water-condensation air sampling for the quantitative measurement of airborne influenza viruses. The measurements, compared with RT-qPCR, demonstrated consistency between the two. In the measurements of airborne influenza viruses conducted in an elementary school using G-AVS, 23% (4/17) of indoor air samples were positive, with concentrations ranging from 1.7 × 10⁴ to 1.6 × 10⁶ gene copies/m³, while losses in the HA relative to NP were 48–75% at a relative humidity of 27.0–36.8% and 60 min air sampling, similar to infectivities reported in the literature. This platform has the potential for rapid and cost-effective airborne virus measurement.

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基于纸质免疫传感器和基于生长的病毒气溶胶浓缩器的空气中流感病毒监测平台

测量室内空气中的呼吸道病毒对于有效预防疾病传播至关重要。通常的方法是对空气采样病毒的核酸或斑块进行计数。在本文中，我们介绍了一种基于生长的空气传播病毒监测（G-AVS）平台，该平台基于纸质电化学免疫传感器，以血凝素（HA）和核蛋白（NP）为目标，采用水凝空气采样法定量测量空气传播的流感病毒。测量结果与 RT-qPCR 相比，两者具有一致性。在使用 G-AVS 对一所小学进行的空气传播流感病毒测量中，23%（4/17）的室内空气样本呈阳性，浓度范围为 1.7 × 104 至 1.6 × 106 基因拷贝/立方米，而在相对湿度为 27.0-36.8% 和 60 分钟空气采样条件下，HA 相对于 NP 的损失率为 48-75%，与文献报道的感染率相似。该平台具有快速、经济高效地测量空气传播病毒的潜力。

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来源期刊

环境科学与技术环境科学-工程：环境

CiteScore

17.50

自引率

9.60%

发文量

12359

审稿时长

2.8 months

期刊介绍： Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences. Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.