Application of a single "Universal warming protocol" for vitrified donor oocytes: A multicenter study.

IF 3.2 3区医学 Q2 GENETICS & HEREDITY

Journal of Assisted Reproduction and Genetics Pub Date : 2025-03-28 DOI:10.1007/s10815-025-03423-7

Paloma Troncoso-Perez, Cristina Gonzalez-Navas, Maria Elisabetta Coccia, Rossella Fucci, Patrizia Falcone, Francesco Bertocci, Rita Picone, Daniel Fernando Sosa-Rosales, Nuria Lopez-Perez, Enrique Criado-Scholz, Miguel Angel Vilches-Ferron

{"title":"Application of a single \"Universal warming protocol\" for vitrified donor oocytes: A multicenter study.","authors":"Paloma Troncoso-Perez, Cristina Gonzalez-Navas, Maria Elisabetta Coccia, Rossella Fucci, Patrizia Falcone, Francesco Bertocci, Rita Picone, Daniel Fernando Sosa-Rosales, Nuria Lopez-Perez, Enrique Criado-Scholz, Miguel Angel Vilches-Ferron","doi":"10.1007/s10815-025-03423-7","DOIUrl":null,"url":null,"abstract":"Purpose: The objective of the multicenter study is to demonstrate the efficacy of the \"Universal Warming Protocol (UWP)\" using different devitrification media in donor oocytes that were previously vitrified in a single egg bank.Method: A total of 2172 oocytes (metaphase II) were vitrified at Ovobank (Spain) using Kitazato vitrification media (Kitazato, Japan). The oocytes were devitrified at three different centers. Group A: 1021 oocytes devitrified with Kitazato media (Kitazato, Japan); Group B: 496 oocytes devitrified with Irvine media (Fujifilm Irvine Scientific, USA); Group C: 655 oocytes devitrified with RapidWarm Omni media (Vitrolife, Sweden). The study parameters included survival rate, fertilization rate, cleavage rate, blastulation rate, and a pregnancy rate confirmed by a biochemical test; transferring embryos at D + 3 and D + 5; and clinical pregnancy rate, transferring embryos at D + 3 and D + 5.Results: The survival rate was statistically higher in Group C (94%) compared to Group A (86%) and Group B (85%) (P value < 0.01). There were no statistically significant differences in the remaining parameters: fertilization rate (A: 77%, B: 74%, C: 73%); cleavage rate (A: 83%, B: 83%, C: 80%); blastulation rate (A: 52%, B: 58%, C: 57%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 3 (A: 38%, B: 33%, C: 33%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 5 (A: 54%, B: 76%, C: 65%); clinical pregnancy rate in transferring embryos at D + 3 (A: 27%, B: 13%, C: 18%); clinical pregnancy rate in transferring embryos at D + 5 (A: 39%, B: 57%, C: 60%).Conclusions: The idea that oocyte devitrification medium should always be from the same supplier is questionable. Therefore, it is feasible to use different devitrification media for donor oocytes that were vitrified with the same vitrification medium.","PeriodicalId":15246,"journal":{"name":"Journal of Assisted Reproduction and Genetics","volume":" ","pages":""},"PeriodicalIF":3.2000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Assisted Reproduction and Genetics","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s10815-025-03423-7","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"GENETICS & HEREDITY","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose: The objective of the multicenter study is to demonstrate the efficacy of the "Universal Warming Protocol (UWP)" using different devitrification media in donor oocytes that were previously vitrified in a single egg bank.

Method: A total of 2172 oocytes (metaphase II) were vitrified at Ovobank (Spain) using Kitazato vitrification media (Kitazato, Japan). The oocytes were devitrified at three different centers. Group A: 1021 oocytes devitrified with Kitazato media (Kitazato, Japan); Group B: 496 oocytes devitrified with Irvine media (Fujifilm Irvine Scientific, USA); Group C: 655 oocytes devitrified with RapidWarm Omni media (Vitrolife, Sweden). The study parameters included survival rate, fertilization rate, cleavage rate, blastulation rate, and a pregnancy rate confirmed by a biochemical test; transferring embryos at D + 3 and D + 5; and clinical pregnancy rate, transferring embryos at D + 3 and D + 5.

Results: The survival rate was statistically higher in Group C (94%) compared to Group A (86%) and Group B (85%) (P value < 0.01). There were no statistically significant differences in the remaining parameters: fertilization rate (A: 77%, B: 74%, C: 73%); cleavage rate (A: 83%, B: 83%, C: 80%); blastulation rate (A: 52%, B: 58%, C: 57%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 3 (A: 38%, B: 33%, C: 33%); a pregnancy rate confirmed by a biochemical test in transferring embryos at D + 5 (A: 54%, B: 76%, C: 65%); clinical pregnancy rate in transferring embryos at D + 3 (A: 27%, B: 13%, C: 18%); clinical pregnancy rate in transferring embryos at D + 5 (A: 39%, B: 57%, C: 60%).

Conclusions: The idea that oocyte devitrification medium should always be from the same supplier is questionable. Therefore, it is feasible to use different devitrification media for donor oocytes that were vitrified with the same vitrification medium.

查看原文本刊更多论文

目的：这项多中心研究的目的是证明 "通用升温方案（UWP）"使用不同脱盐培养基对先前在单一卵子库进行玻璃化的供体卵母细胞的有效性：方法：Ovobank（西班牙）使用 Kitazato 玻璃化培养基（日本 Kitazato）对 2172 个卵母细胞（分裂期 II）进行了玻璃化。卵母细胞在三个不同的中心进行玻璃化。A 组：1021 个卵母细胞使用 Kitazato 培养基（Kitazato，日本）进行玻璃化；B 组：496 个卵母细胞使用 Irvine 培养基（Fujifilm Irvine Scientific，美国）进行玻璃化；C 组：655 个卵母细胞使用 RapidWarm Omni 培养基（Vitrolife，瑞典）进行玻璃化。研究参数包括存活率、受精率、卵裂率、胚胎着床率和经生化测试确认的妊娠率；在 D + 3 和 D + 5 时移植胚胎；以及在 D + 3 和 D + 5 时移植胚胎的临床妊娠率：结果：与 A 组（86%）和 B 组（85%）相比，C 组（94%）的存活率在统计学上更高（P 值）：卵母细胞脱盐培养基应始终来自同一供应商的观点值得商榷。因此，对使用相同玻璃化培养基进行玻璃化的供体卵母细胞使用不同的玻璃化培养基是可行的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Assisted Reproduction and Genetics 医学-妇产科学

CiteScore

5.70

自引率

9.70%

发文量

286

审稿时长

1 months

期刊介绍： The Journal of Assisted Reproduction and Genetics publishes cellular, molecular, genetic, and epigenetic discoveries advancing our understanding of the biology and underlying mechanisms from gametogenesis to offspring health. Special emphasis is placed on the practice and evolution of assisted reproduction technologies (ARTs) with reference to the diagnosis and management of diseases affecting fertility. Our goal is to educate our readership in the translation of basic and clinical discoveries made from human or relevant animal models to the safe and efficacious practice of human ARTs. The scientific rigor and ethical standards embraced by the JARG editorial team ensures a broad international base of expertise guiding the marriage of contemporary clinical research paradigms with basic science discovery. JARG publishes original papers, minireviews, case reports, and opinion pieces often combined into special topic issues that will educate clinicians and scientists with interests in the mechanisms of human development that bear on the treatment of infertility and emerging innovations in human ARTs. The guiding principles of male and female reproductive health impacting pre- and post-conceptional viability and developmental potential are emphasized within the purview of human reproductive health in current and future generations of our species. The journal is published in cooperation with the American Society for Reproductive Medicine, an organization of more than 8,000 physicians, researchers, nurses, technicians and other professionals dedicated to advancing knowledge and expertise in reproductive biology.