Mumia qirimensis sp. nov. isolated from the rhizosphere of Phyllostachys viridiglaucescens (Carrière) Rivière & C. Rivière.

Abstract

A novel actinomycete strain, designated Pv 4-285^T, was isolated from rhizosphere sample collected from Phyllostachys viridiglaucescens growing in the Nikitsky Botanical Garden, Crimea, Ukraine. The strain Pv 4-285 ^T shared the highest 16S rRNA gene sequence similarity with Mumia xiangluensis NEAU-KD1^T. The genome size was 4.6 Mbp with a DNA G+C content of 70.43%. The isolate was Gram-stain-positive, non-spore-forming, non-motile and aerobic. Cells were observed to be irregularly rod-shaped. Pv 4-285^T was able to grow at 20-37 °C and at pH 6.0-9.0 and in the presence of up to 6% (w/v) NaCl. The predominant menaquinone was detected as MK-9(H₄). The polar lipid profile of strain Pv 4-285^T consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol mannoside and phosphatidylinositol. Pv 4-285 contained C_16:0, С_18:1 ω9c, 10-methyl C_18:0, C_17:0. While galactose, mannose and xylose were detected in strain Pv 4-285^T as the whole-cell sugars. Based on these differences, strain Pv 4-285^T (=DSM 118545^T =LMG 33743^T) should be classified as the type strain of a novel species of Mumia, for which the name Mumia qirimensis sp. nov. is proposed.