{"title":"Synthesis of a Quinoline-Coumarin Fluorescent Probe and its Applications.","authors":"Mingya Wang, Junli Shi, Duanlin Cao, Linxiu Zhao","doi":"10.1007/s10895-025-04257-3","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, probe T is synthesized via a condensation reaction from 7-diethylamino-3-formylcoumarin and 2-(quinolin-8-yloxy) acetohydrazide. It demonstrates a strong recognition ability for Cu<sup>2+</sup> and S<sup>2-</sup> in the CH<sub>3</sub>CN/HEPES (9:1, V/V, pH = 7.34) buffer system. Upon the addition of Cu<sup>2+</sup>, the probe solution undergoes a visible color change from yellow-green to brown-yellow, with a noticeable red shift of 30 nm in the maximum absorption wavelength. Under UV light, the solution fluorescence is significantly quenched, with a quenching rate of 98.6% and a detection limit of 0.289 µmol/L. The complex T-Cu<sup>2+</sup> formed by probe T and Cu<sup>2+</sup> enables continuous recognition of S<sup>2-</sup>, demonstrating a fluorescence recovery phenomenon with a recovery rate of 88.9% and a detection limit of 0.01 µmol/L. Probe T demonstrates excellent recognition ability for Cu<sup>2+</sup> and S<sup>2-</sup> at pH 4 ~ 9 and can be recycled for 4 times. Additionally, probe T was effectively employed in fabricating test strips for the rapid and convenient detection of Cu<sup>2+</sup> and S<sup>2-</sup>, and for determining Cu<sup>2+</sup> and S<sup>2-</sup> in living cells, owing to its excellent permeability and low cytotoxicity.</p>","PeriodicalId":15800,"journal":{"name":"Journal of Fluorescence","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Fluorescence","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1007/s10895-025-04257-3","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, probe T is synthesized via a condensation reaction from 7-diethylamino-3-formylcoumarin and 2-(quinolin-8-yloxy) acetohydrazide. It demonstrates a strong recognition ability for Cu2+ and S2- in the CH3CN/HEPES (9:1, V/V, pH = 7.34) buffer system. Upon the addition of Cu2+, the probe solution undergoes a visible color change from yellow-green to brown-yellow, with a noticeable red shift of 30 nm in the maximum absorption wavelength. Under UV light, the solution fluorescence is significantly quenched, with a quenching rate of 98.6% and a detection limit of 0.289 µmol/L. The complex T-Cu2+ formed by probe T and Cu2+ enables continuous recognition of S2-, demonstrating a fluorescence recovery phenomenon with a recovery rate of 88.9% and a detection limit of 0.01 µmol/L. Probe T demonstrates excellent recognition ability for Cu2+ and S2- at pH 4 ~ 9 and can be recycled for 4 times. Additionally, probe T was effectively employed in fabricating test strips for the rapid and convenient detection of Cu2+ and S2-, and for determining Cu2+ and S2- in living cells, owing to its excellent permeability and low cytotoxicity.
期刊介绍:
Journal of Fluorescence is an international forum for the publication of peer-reviewed original articles that advance the practice of this established spectroscopic technique. Topics covered include advances in theory/and or data analysis, studies of the photophysics of aromatic molecules, solvent, and environmental effects, development of stationary or time-resolved measurements, advances in fluorescence microscopy, imaging, photobleaching/recovery measurements, and/or phosphorescence for studies of cell biology, chemical biology and the advanced uses of fluorescence in flow cytometry/analysis, immunology, high throughput screening/drug discovery, DNA sequencing/arrays, genomics and proteomics. Typical applications might include studies of macromolecular dynamics and conformation, intracellular chemistry, and gene expression. The journal also publishes papers that describe the synthesis and characterization of new fluorophores, particularly those displaying unique sensitivities and/or optical properties. In addition to original articles, the Journal also publishes reviews, rapid communications, short communications, letters to the editor, topical news articles, and technical and design notes.