Co-Cultivation with Mesenchymal Stem Cells Does Not Affect the Efficiency of DNA Double-Strand Break Repair in Irradiated Human Non-Small Cell Lung Cancer Cells.
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Abstract
The effect of the microenvironment on the efficiency of anti-cancer therapy is one of the most discussed topics in modern oncology. In this study, we evaluated the effect of co-cultivation with human mesenchymal stem cells (MSCs) on the efficiency of DNA double-strand break (DSB) repair in human non-small cell lung cancer (NSCLC) A549 cell line exposed to X-ray radiation. In addition, the effect of co-cultivation of MSCs and A549 cells on the proliferative activity of non-irradiated NSCLC was also studied. To assess the efficiency of DNA DSB repair, we analyzed the quantitative yield of residual foci of DSB marker proteins (γH2AX and 53BP1) 24 h after irradiation with doses of 2, 4, and 6 Gy. The results showed that co-cultivation with MSCs did not affect the efficiency of DNA DSB repair induced by X-rays, as well as the proliferative activity of NSCLC cells.
期刊介绍：
Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005.
World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells.
The Russian Volume Year is published in English from April.
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