Haidy Sayed Rashad, Hesham A. Nada, Nader Ali Ismail, Noha M. Abd El-Fadeal, Nora Hosny, Mona A. Atwa
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引用次数: 0
Abstract
The pilosebaceous unit is the site of a common chronic inflammatory skin disorder called acne. Tight junctions (TJ) are present in sebocytes and are primarily composed of claudin-1. When claudin-1 is knocked down, the TJ barrier leaks, and holocrine secretion is compromised. This leads to the accumulation of partially degenerated sebocytes in the pilosebaceous ducts and the creation of microcomedos, which can cause acne lesions. The study’s aim was to measure the claudin-1 gene and protein expression in acne patients’ blood samples and establish a correlation with the severity of the condition and their levels. 48 acne patients were involved and 48 matched control for age and sex. First samples were obtained from both groups and RNA was extracted from the whole blood sample followed by qPCR to measure the relative expression of the claudin-1 gene. Enzyme-linked immunosorbent assay (ELISA) was used to measure claudin-1 protein level. There were statistically significant lower relative expression of claudin-1 gene (p = 0.001) and its protein level (p = 0.001) in acne patients compared to controls (0.34 ± 0.32 vs 1.0 ± 0.0, 0.64 ± 0.62 vs 3.85 ± 2.14, respectively). There was a statistically significant inverse correlation between claudin-1 protein expression and acne severity (R = −0.374, p = 0.009). Computational and bioinformatics tools were used for enrichment analysis to better understand claudin-1 role in the context of the disease and provide possible therapeutic approaches. Claudin-1 dysregulation in acne patients, suggest that it have an impact on the severity and course of acne through affecting the epidermal barrier and facilitating inflammation.
皮脂腺单位是一种常见的慢性炎症性皮肤疾病,称为痤疮的部位。紧密连接(TJ)存在于脂细胞中,主要由claudin-1组成。当claudin-1被破坏时,TJ屏障泄漏,全息分泌受损。这导致皮脂腺导管中部分退化的皮脂腺细胞的积累和微粉刺的产生,这可能导致痤疮病变。该研究的目的是测量痤疮患者血液样本中claudin-1基因和蛋白质的表达,并建立与病情严重程度及其水平的相关性。48名痤疮患者参与其中,48名年龄和性别匹配的对照组。两组均先取标本,全血提取RNA,采用qPCR检测claudin-1基因的相对表达。采用酶联免疫吸附试验(ELISA)检测claudin-1蛋白水平。痤疮患者claudin-1基因相对表达量(p = 0.001)和蛋白水平(p = 0.001)均低于对照组(分别为0.34±0.32 vs 1.0±0.0、0.64±0.62 vs 3.85±2.14)。claudin-1蛋白表达与痤疮严重程度呈显著负相关(R = - 0.374, p = 0.009)。利用计算和生物信息学工具进行富集分析,以更好地了解claudin-1在疾病背景下的作用,并提供可能的治疗方法。痤疮患者的Claudin-1失调表明它通过影响表皮屏障和促进炎症对痤疮的严重程度和病程有影响。
期刊介绍:
Archives of Dermatological Research is a highly rated international journal that publishes original contributions in the field of experimental dermatology, including papers on biochemistry, morphology and immunology of the skin. The journal is among the few not related to dermatological associations or belonging to respective societies which guarantees complete independence. This English-language journal also offers a platform for review articles in areas of interest for dermatologists and for publication of innovative clinical trials.