A simple colorimetric and paper-based-smartphone sensing platform based on the enhanced peroxidase-like activity of Al doping Prussian blue for point-of-care detection of GSH

IF 6.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2025-03-30 DOI:10.1016/j.talanta.2025.128020

Ying Yang , Liuyan Wei , Weiqin You, Huisha Huang, Shuai Wang, Dandan Hou, Chujun Qin, Zhiheng Su, Mei Li

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Abstract

Glutathione (GSH) is a very important antioxidant and also participates in many important physiological processes, accurate determination of GSH in food and blood fluid is crucial for human health. Herein, we introduce a simple colorimetric and paper-based-smartphone dual signal output platform for point-of-care (POC) detection of GSH in garlic and human serum. The experiment revealed that the peroxidase-like activity of Aluminum-doped Prussian blue (AlPB) could be enhanced by the doping of Al element on Prussian blue. AlPB exhibited excellent peroxidase-like activity and efficiently catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) in the presence of H₂O₂ to generate blue oxidized TMB, resulting in an absorbance and color-based-smartphone dual signal readouts. Enzyme kinetic studies also indicated that the AlPB showed high affinity towards TMB. Hence, a reliable colorimetric and color-based-smartphone dual signal readouts assay was constructed based on AlPB-mediated the peroxidase-like activity, it was used for highly sensitive colorimetric and POC detection of GSH in food and human serum within 7 min with a wide range of linear response from 1 to 20 μM, a low detection limit of 0.1748 μM. This work demonstrates a novel and versatile strategy to develop superior peroxidase mimics and holds great potential for rapid and portable detection of GSH in food, healthcare and clinical diagnosis, and also open promising avenues for more powerful paper-based POC tests.

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一个简单的比色和基于纸张的智能手机传感平台，该平台基于增强的过氧化物酶样活性的Al掺杂普鲁士蓝，用于GSH的即时检测

谷胱甘肽（GSH）是一种非常重要的抗氧化剂，参与许多重要的生理过程，准确测定食物和血液中谷胱甘肽的含量对人体健康至关重要。在此，我们介绍了一种简单的比色法和基于纸张的智能手机双信号输出平台，用于即时检测大蒜和人血清中的谷胱甘肽。实验结果表明，铝掺杂普鲁士蓝（AlPB）可以增强其过氧化物酶样活性。AlPB表现出优异的过氧化物酶样活性，在H2O2存在下有效催化3,3 ',5,5 ' -四甲基联苯胺（TMB）氧化生成蓝色氧化TMB，导致吸光度和基于颜色的智能手机双信号读出。酶动力学研究也表明AlPB对TMB具有较高的亲和力。因此，基于alpb介导的过氧化物酶样活性，构建了一种可靠的比色和基于颜色的智能手机双信号检测方法，该方法可在7 min内对食品和人血清中的谷胱甘肽进行高灵敏度的比色和POC检测，线性响应范围为1 ~ 20 μM，检测限为0.1748 μM。这项工作展示了一种新的和通用的策略来开发卓越的过氧化物酶模拟物，并在食品，医疗保健和临床诊断中快速和便携式检测谷胱甘肽方面具有巨大的潜力，也为更强大的纸质POC测试开辟了有希望的途径。

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.