Investigation of the main active ingredients of Vitex rotundifolia L.f and assessment of their contribution to the activity of this species

Bich Thao Lam , Hai Trieu Ly , Huyen Tran Tran , Van Minh Le
{"title":"Investigation of the main active ingredients of Vitex rotundifolia L.f and assessment of their contribution to the activity of this species","authors":"Bich Thao Lam ,&nbsp;Hai Trieu Ly ,&nbsp;Huyen Tran Tran ,&nbsp;Van Minh Le","doi":"10.1016/j.prmcm.2025.100608","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Quality control of medicinal materials before research and use is essential. <em>Vitex rotundifolia</em> L.f. (also called Manjingzi in traditional Chinese medicine) is a medicinal plant with high medicinal value and uses, which is also prioritized for development in Vietnam. This study aimed to develop a validated high-performance liquid chromatography (HPLC) method for the simultaneous quantification of major compounds in <em>V. rotundifolia</em> for preparing the potential extract and evaluate the pharmacological effects of the <em>V. rotundifolia</em> extract and essential oils.</div></div><div><h3>Methods</h3><div>HPLC method was applied for the determination of two major active compounds in <em>V. rotundifolia</em>, agnuside and casticin, which were determined according to the guidelines of the International Conference on Harmonization (ICH) and Association of Official Analytical Chemists (AOAC). The constituents of <em>V. rotundifolia</em> essential oils were also identified by gas chromatography-mass spectrometry (GC–MS). Pharmacological effects were evaluate via <em>in vitro</em> and <em>in vivo</em> experiments.</div></div><div><h3>Results</h3><div>The validated method demonstrated acceptable precision and was used to analyze the concentrations of agnuside and casticin in different extracts from different collection conditions of <em>V. rotundifolia</em>, the 70 % ethanol extract of twig and leaf <em>V. rotundifolia</em> collected in Ninh Hai district at noon and shade-dried was selected as a potential extract. Interestingly, the pharmacological effects of the potential extract containing agnuside and casticin were demonstrated including anti-inflammatory, analgesic, anticancer, antioxidant, and antibacterial activities. Furthermore, <em>V. rotundifolia</em> essential oils contain major constituents of α-pinene, sabinene, β-pinene, eucalyptol, and β-terpinyl acetate with confirmed antibacterial activity.</div></div><div><h3>Discussion</h3><div>This study has evaluated relatively comprehensively <em>Vitex rotundifolia</em> L.f. from chemical composition, quantitative method to biological effects, showing that this is a potential medicinal plant that can be developed in the near future.</div></div>","PeriodicalId":101013,"journal":{"name":"Pharmacological Research - Modern Chinese Medicine","volume":"15 ","pages":"Article 100608"},"PeriodicalIF":0.0000,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmacological Research - Modern Chinese Medicine","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667142525000375","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Introduction

Quality control of medicinal materials before research and use is essential. Vitex rotundifolia L.f. (also called Manjingzi in traditional Chinese medicine) is a medicinal plant with high medicinal value and uses, which is also prioritized for development in Vietnam. This study aimed to develop a validated high-performance liquid chromatography (HPLC) method for the simultaneous quantification of major compounds in V. rotundifolia for preparing the potential extract and evaluate the pharmacological effects of the V. rotundifolia extract and essential oils.

Methods

HPLC method was applied for the determination of two major active compounds in V. rotundifolia, agnuside and casticin, which were determined according to the guidelines of the International Conference on Harmonization (ICH) and Association of Official Analytical Chemists (AOAC). The constituents of V. rotundifolia essential oils were also identified by gas chromatography-mass spectrometry (GC–MS). Pharmacological effects were evaluate via in vitro and in vivo experiments.

Results

The validated method demonstrated acceptable precision and was used to analyze the concentrations of agnuside and casticin in different extracts from different collection conditions of V. rotundifolia, the 70 % ethanol extract of twig and leaf V. rotundifolia collected in Ninh Hai district at noon and shade-dried was selected as a potential extract. Interestingly, the pharmacological effects of the potential extract containing agnuside and casticin were demonstrated including anti-inflammatory, analgesic, anticancer, antioxidant, and antibacterial activities. Furthermore, V. rotundifolia essential oils contain major constituents of α-pinene, sabinene, β-pinene, eucalyptol, and β-terpinyl acetate with confirmed antibacterial activity.

Discussion

This study has evaluated relatively comprehensively Vitex rotundifolia L.f. from chemical composition, quantitative method to biological effects, showing that this is a potential medicinal plant that can be developed in the near future.

Abstract Image

调查蔓荆子的主要活性成分并评估其对该物种活性的贡献
引言 在研究和使用前对药材进行质量控制至关重要。蔓荆子(Vitex rotundifolia L.f.,中药中又称蔓荆子)是一种具有很高药用价值和用途的药用植物,也是越南优先发展的药用植物。本研究旨在开发一种有效的高效液相色谱(HPLC)方法,用于同时定量分析旋覆花(V. rotundifolia)中的主要化合物,以制备潜在的提取物,并评估旋覆花提取物和精油的药理作用。方法 采用 HPLC 方法测定旋覆花中的两种主要活性化合物--龙葵甙和诃子苷,其测定方法符合国际协调会议(ICH)和官方分析化学家协会(AOAC)的指导原则。此外,还采用气相色谱-质谱法(GC-MS)对香叶精油的成分进行了鉴定。结果该方法的精密度可以接受,并被用于分析不同采集条件下罗汉果提取物中芒果苷和肉豆蔻苷的浓度,其中宁海县正午采集并阴干的罗汉果树枝和叶的 70% 乙醇提取物被选为潜在提取物。有趣的是,含有龙脑内酯和木犀草苷的潜在提取物的药理作用得到了证实,包括抗炎、镇痛、抗癌、抗氧化和抗菌活性。讨论本研究从化学成分、定量方法到生物效应等方面,对蔓越橘(Vitex rotundifolia L.f.)进行了较为全面的评估,表明它是一种具有潜力的药用植物,在不久的将来可以得到开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
1.60
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信