Multivariate analysis of potentially toxic metal contents in soil and vegetables: Enrichment, bioconcentration, translocation from soil to vegetables, and assessment of human health toxicity

Abstract

The excessive accumulation of metals in agricultural soils can profoundly impact the quality of vegetables grown in contaminated soil. Understanding the bioaccumulation of these metals in vegetables is essential for assessing human exposure risks. The present study aimed to investigate the concentration of potentially toxic metals (PTMs: Fe, Mn, Cu, Zn, Al, As, Cr, Cd, and Pb) in agricultural soil and some commonly consumed vegetables (carrot, reddish, cauliflower, pumpkin, and spinach). The samples were collected from agrarian farmlands near the industrial area of Multan Road, Kasur-Pakistan. The mean contents of all metals in soil, root, and shoot samples were within the limits set by the EU, WHO, FAO, and US EPA, except for Cr in soil and Cr, Cd, and Pb in root samples. Across all analyzed vegetables, the bioconcentration factors (BCF_root and BCF_shoot < 1, except As) and translocation factor (TF < 1) for all metals suggested that while these vegetables absorb metals, they generally do not accumulate or translocate them. Multivariate analysis indicated that both natural and anthropogenic activities contribute to metal contamination. The calculated hazard index (HI > 1) and cumulative cancer risk (CCR >1 × 10⁻³) values indicated the probability of non-carcinogenic and carcinogenic health risks for adults and children associated with the consumption of these vegetables. The findings provide critical insights for policymakers, agricultural regulators, and public health authorities to mitigate metal contamination risks and promote food safety.