Exploration of cobalt-based colorimetric aptasensing of zearalenone in cereal products: Enhanced performance of Au/CoOOH nanozyme

IF 6 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

LWT - Food Science and Technology Pub Date : 2025-03-26 DOI:10.1016/j.lwt.2025.117700

Qi Sun , Miaomiao Ma , Zhijuan Li , Xiang Chen , Jian Zeng , Meiqi Huang , Yuting Yin , Xueyan Ran , Jianmei Li , Tao Le

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引用次数: 0

Abstract

Zearalenone (ZEN) poses significant threats to food safety and human health due to its widespread contamination in cereals. Current detection methods face limitations in cost, complexity, and field applicability. To address this, we developed a novel colorimetric aptasensor leveraging the peroxidase-like activity of Au/CoOOH nanozymes for rapid and sensitive ZEN detection. Systematic characterization of cobalt-based nanomaterials (β-Co(OH)₂, Co₃O₄, CoOOH, and Au/CoOOH) revealed that Au/CoOOH exhibited the highest catalytic efficiency, achieving a 3.5-fold enhancement over CoOOH due to synergistic interactions with Au nanoparticles. The aptasensor employed a competitive displacement strategy: ZEN binding to aptamer-functionalized magnetic beads released cDNA-conjugated Au/CoOOH, reducing 3,3′,5,5′-tetramethylbenzidine oxidation signals. Optimized parameters enabled a linear detection range of 0.3–500 ng/mL with a detection limit of 0.23 ng/mL. Specificity tests confirmed minimal cross-reactivity with other mycotoxins. The sensor demonstrated exceptional sensitivity, specificity, and practicality, validated by recovery rates of 90.65–105.63 % in spiked matrices, positioning it as a reliable tool for safeguarding food safety through rapid, precise mycotoxin monitoring.

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来源期刊

LWT - Food Science and Technology 工程技术-食品科技

CiteScore

11.80

自引率

6.70%

发文量

1724

审稿时长

65 days

期刊介绍： LWT - Food Science and Technology is an international journal that publishes innovative papers in the fields of food chemistry, biochemistry, microbiology, technology and nutrition. The work described should be innovative either in the approach or in the methods used. The significance of the results either for the science community or for the food industry must also be specified. Contributions written in English are welcomed in the form of review articles, short reviews, research papers, and research notes. Papers featuring animal trials and cell cultures are outside the scope of the journal and will not be considered for publication.