Shufen Huo, Heng Zhang, Xiang Li, Xuan Li, Wenli Shang, Sen Sheng, Yingxuan Tian
{"title":"Regulatory interplay between lncRNA-FGD5-AS1 and miR-17-5p in non-small cell lung cancer progression: Implications for novel therapeutic strategies.","authors":"Shufen Huo, Heng Zhang, Xiang Li, Xuan Li, Wenli Shang, Sen Sheng, Yingxuan Tian","doi":"10.1177/09287329251325336","DOIUrl":null,"url":null,"abstract":"<p><p>BackgroundMicroRNA-17-5p (miR-17-5p) plays a pivotal role in the tumorigenesis and progression of non-small cell lung cancer (NSCLC) by regulating its target genes. Advances in molecular biology highlight the importance of long non-coding RNAs (lncRNAs) in cancer, yet the mechanistic interactions between miR-17-5p and lncRNAs in NSCLC remain underexplored.ObjectiveThis study investigated the regulatory interplay between miR-17-5p and lncRNA-FGD5-AS1 and evaluated their potential as targets for NSCLC therapy.MethodsA comprehensive set of technologies, including cell transfection, quantitative real-time PCR (qRT-PCR), bioinformatics analysis, and functional assays (proliferation, migration, apoptosis), was employed to examine the role of miR-17-5p and lncRNA-FGD5-AS1 in NSCLC.ResultsElevated lncRNA-FGD5-AS1 expression was observed in NSCLC cell lines A549 and H1299, correlating with poor patient prognosis. Functional assays revealed that miR-17-5p directly downregulates lncRNA-FGD5-AS1, thereby modulating key oncogenic processes. Overexpression of miR-17-5p reduced tumor cell proliferation and migration while inducing apoptosis. Conversely, miR-17-5p inhibition elevated lncRNA-FGD5-AS1 levels and reversed these effects.ConclusionThe findings identify the miR-17-5p/lncRNA-FGD5-AS1 regulatory axis as a novel therapeutic target for NSCLC. By integrating molecular and technological approaches, this study offers insights into precision oncology and highlights the potential for advanced RNA-based interventions.</p>","PeriodicalId":48978,"journal":{"name":"Technology and Health Care","volume":" ","pages":"9287329251325336"},"PeriodicalIF":1.4000,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Technology and Health Care","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/09287329251325336","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, BIOMEDICAL","Score":null,"Total":0}
引用次数: 0
Abstract
BackgroundMicroRNA-17-5p (miR-17-5p) plays a pivotal role in the tumorigenesis and progression of non-small cell lung cancer (NSCLC) by regulating its target genes. Advances in molecular biology highlight the importance of long non-coding RNAs (lncRNAs) in cancer, yet the mechanistic interactions between miR-17-5p and lncRNAs in NSCLC remain underexplored.ObjectiveThis study investigated the regulatory interplay between miR-17-5p and lncRNA-FGD5-AS1 and evaluated their potential as targets for NSCLC therapy.MethodsA comprehensive set of technologies, including cell transfection, quantitative real-time PCR (qRT-PCR), bioinformatics analysis, and functional assays (proliferation, migration, apoptosis), was employed to examine the role of miR-17-5p and lncRNA-FGD5-AS1 in NSCLC.ResultsElevated lncRNA-FGD5-AS1 expression was observed in NSCLC cell lines A549 and H1299, correlating with poor patient prognosis. Functional assays revealed that miR-17-5p directly downregulates lncRNA-FGD5-AS1, thereby modulating key oncogenic processes. Overexpression of miR-17-5p reduced tumor cell proliferation and migration while inducing apoptosis. Conversely, miR-17-5p inhibition elevated lncRNA-FGD5-AS1 levels and reversed these effects.ConclusionThe findings identify the miR-17-5p/lncRNA-FGD5-AS1 regulatory axis as a novel therapeutic target for NSCLC. By integrating molecular and technological approaches, this study offers insights into precision oncology and highlights the potential for advanced RNA-based interventions.
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Technology and Health Care is intended to serve as a forum for the presentation of original articles and technical notes, observing rigorous scientific standards. Furthermore, upon invitation, reviews, tutorials, discussion papers and minisymposia are featured. The main focus of THC is related to the overlapping areas of engineering and medicine. The following types of contributions are considered:
1.Original articles: New concepts, procedures and devices associated with the use of technology in medical research and clinical practice are presented to a readership with a widespread background in engineering and/or medicine. In particular, the clinical benefit deriving from the application of engineering methods and devices in clinical medicine should be demonstrated. Typically, full length original contributions have a length of 4000 words, thereby taking duly into account figures and tables.
2.Technical Notes and Short Communications: Technical Notes relate to novel technical developments with relevance for clinical medicine. In Short Communications, clinical applications are shortly described. 3.Both Technical Notes and Short Communications typically have a length of 1500 words.
Reviews and Tutorials (upon invitation only): Tutorial and educational articles for persons with a primarily medical background on principles of engineering with particular significance for biomedical applications and vice versa are presented. The Editorial Board is responsible for the selection of topics.
4.Minisymposia (upon invitation only): Under the leadership of a Special Editor, controversial or important issues relating to health care are highlighted and discussed by various authors.
5.Letters to the Editors: Discussions or short statements (not indexed).
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