Macromolecular crystallography at Elettra: current and future perspectives.

IF 2.5 3区物理与天体物理

Journal of Synchrotron Radiation Pub Date : 2025-05-01 Epub Date: 2025-03-26 DOI:10.1107/S1600577525001055

Raghurama P Hegde, Nicola Demitri, Annie Héroux, Alessandro Olivo, Giorgio Bais, Michele Cianci, Paola Storici, Dan George Dumitrescu, Nishant Kumar Varshney, Balasubramanian Gopal, D D Sarma, Lisa Vaccari, Silvia Onesti, Maurizio Polentarutti

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引用次数: 0

Abstract

The Elettra synchrotron radiation facility, located in Trieste, Italy, is a third-generation storage ring, operating in top-up mode at both 2.0 and 2.4 GeV. The facility currently hosts one beamline fully dedicated to macromolecular crystallography, XRD2. XRD2 is based on a superconducting wiggler, and it has been open to users since 2018. On-site and remote access for data collection, as well as monitoring tools and automatic data analysis pipelines are available to its users. In addition, since 1994 Elettra has operated a general-purpose diffraction beamline, XRD1, offering the macromolecular community a wide spectrum extending to long wavelengths for phasing and ion identification. Ancillary facilities support the beamlines, providing sample preparation and a high-throughput crystallization platform for the user community. A new CryoEM facility is being established on campus and jointly operated by the Consiglio Nazionale della Ricerche - Istituto Officina dei Materiali (CNR-IOM) and Elettra, providing further opportunities to the Elettra user community. This review outlines the current capabilities and anticipated developments for macromolecular crystallography at Elettra to accompany the upcoming upgrade to Elettra 2.0, featuring a six-bend enhanced achromat lattice. The new source is expected to deliver a high-brilliance beam, enabling the macromolecular crystallography community to better address the emerging and future scientific challenges.

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Elettra的大分子晶体学：当前和未来的观点。

Elettra同步辐射设施位于意大利的里雅斯特，是第三代存储环，在2.0和2.4 GeV的充值模式下运行。该设施目前拥有一条完全专用于大分子晶体学的光束线，XRD2。XRD2基于超导摆动器，自2018年以来一直向用户开放。用户可以使用现场和远程访问数据收集，以及监控工具和自动数据分析管道。此外，自1994年以来，Elettra已经运营了通用衍射光束线XRD1，为大分子社区提供了延伸到长波长的宽光谱，用于相位和离子识别。辅助设施支持光束线，为用户社区提供样品制备和高通量结晶平台。一个新的CryoEM设施正在校园内建立，由CNR-IOM和Elettra联合运营，为Elettra用户社区提供了更多的机会。这篇综述概述了Elettra大分子晶体学目前的能力和预期的发展，伴随着即将升级到Elettra 2.0，具有六弯增强的消色差晶格。新的光源有望提供高亮度光束，使大分子晶体学界能够更好地应对新兴和未来的科学挑战。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Synchrotron Radiation INSTRUMENTS & INSTRUMENTATIONOPTICS&-OPTICS

CiteScore

5.60

自引率

12.00%

发文量

289

审稿时长

1 months

期刊介绍： Synchrotron radiation research is rapidly expanding with many new sources of radiation being created globally. Synchrotron radiation plays a leading role in pure science and in emerging technologies. The Journal of Synchrotron Radiation provides comprehensive coverage of the entire field of synchrotron radiation and free-electron laser research including instrumentation, theory, computing and scientific applications in areas such as biology, nanoscience and materials science. Rapid publication ensures an up-to-date information resource for scientists and engineers in the field.