The capacity of the green microalga Chlorella vulgaris in overcoming the detrimental effects of cephalexin contamination.

Abstract

Antibiotics have the potential to affect the health of humans and other living organisms even at slight concentrations. Therefore, there has been a growing global awareness of the environmental impacts associated with antibiotics as emerging pollutants. Cephalexin (CPX) is classified as a first-generation cephalosporin and exhibits a significant efficacy in combating bacterial infections. The current work was conducted to examine the capability of the microalga Chlorella vulgaris to mitigate CPX contamination in aquatic environments. The results indicated that the growth of microalgae diminished in a dose-dependent manner after a 6-day exposure to concentrations of 200-800 mg L^- 1 CPX. The analysis conducted through scanning electron microscopy revealed alterations in cell morphology, specifically shrinkage and wrinkling, following the application of CPX. These effects became more significant as the concentration of CPX increased. The results from flow cytometry revealed a notable decrease in cell viability for all concentrations of CPX used, with the highest concentration yielding a viability rate of less than 30%. In addition, CPX caused a decrease in levels of photosynthetic pigments and non-enzymatic antioxidants, including phenols and flavonoids. However, the activity levels of the main antioxidant enzymes considerably increased, achieving their peak at 800 mg L⁻¹. Moreover, the algal cells demonstrated the capability to decrease the concentration of CPX present in the contaminated media, with the most effective reduction observed at 400 mg L^- 1. The data obtained confirmed the significant toxicity of CPX on Chlorella vulgaris, while also emphasizing the ability of microalgal cells to withstand antibiotic contamination.