Exploring the occurrence of Pseudomonas aeruginosa and comprehensive whole genome analysis of the bcsir_p4_s20 strain from municipal wastewater in Chattogram.

IF 4 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

World journal of microbiology & biotechnology Pub Date : 2025-03-28 DOI:10.1007/s11274-025-04328-4

Md Nuruddin Mahmud, Momthahena Momoshed, Md Faisal Ahamed Talukder, Jannatul Ferdous, Farjana Akter Koly, Saiful Islam

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Abstract

Various studies reported the existence of multidrug-resistant (MDR) Pseudomonas aeruginosa in environmental samples, including hospital wastewater, municipal wastewater, and surface water. In this study, we investigated the impact of untreated municipal wastewater transmitting antibiotic-resistant P. aeruginosa strains in wastewater networks of Chattogram City, Bangladesh, through antibiotic susceptibility profiles and whole-genome sequencing (WGS) of the MDR P. aeruginosa bcsir_p4_s20. Forty-two P. aeruginosa isolates were identified from eight locations using polymerase chain reaction (PCR), targeting the oprI and oprL genes, and antibiotic susceptibility was determined against 11 antibiotics by the disc diffusion method. Resistant isolates were identified at all locations, with the highest resistance frequency displayed towards meropenem, cefepime, and colistin. The WGS of bcsir_p4_s20 was performed using the NextSeq 2000 platform. Several bioinformatics tools, like FastQC, Trimmomatic, SPAdes, and Prokka, were used for quality evaluation, low-quality read and adapter filtration, de novo assembly, and functional annotation. Comprehensive Antibiotic Resistance Database (CARD), AMRFinderPlus, and virulence factor database (VFDB) were employed to determine resistance genes and virulence factors. The strain belongs to the O7 serogroup and sequence type ST357. The analysis identified antibiotic resistance genes (blaPDC-11, sul1, and others) that cause resistance through efflux pump and inactivation mechanisms, and virulent genes responsible for adherence (flagella, type IV pili), enzyme (phospholipase C), iron uptake (pyoverdine), secretion system (exoT, exoU), and toxin (toxA) secretion. Therefore, municipal wastewater is a potential reservoir for MDR P. aeruginosa, and establishing wastewater treatment plants (WWTPs) at the primary source points before discharging it to the wastewater network is suggested to mitigate the risk of outbreaks.

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探讨铜绿假单胞菌的发生，并对城市污水中bcsir_p4_s20菌株进行全基因组分析。

多项研究报道了环境样本中存在多重耐药（MDR）铜绿假单胞菌，包括医院废水、城市废水和地表水。在这项研究中，我们通过抗生素敏感性分析和耐多药铜绿假单胞菌bcsir_p4_s20的全基因组测序（WGS），研究了未经处理的城市污水在孟加拉国Chattogram市废水网络中传播耐药铜绿假单胞菌菌株的影响。采用聚合酶链反应（PCR）技术从8个地点鉴定出42株铜绿假单胞菌，分别靶向oprI和oprL基因，并采用纸片扩散法对11种抗生素进行药敏试验。在所有地点均发现耐药分离株，耐药频率最高的是美罗培南、头孢吡肟和粘菌素。bcsir_p4_s20的WGS采用NextSeq 2000平台进行。一些生物信息学工具，如FastQC、Trimmomatic、SPAdes和Prokka，用于质量评估、低质量读取和适配器过滤、从头组装和功能注释。采用综合抗生素耐药数据库（CARD）、AMRFinderPlus和毒力因子数据库（VFDB）检测耐药基因和毒力因子。该菌株属于O7血清群，序列型为ST357。分析确定了通过外排泵和失活机制引起耐药性的抗生素抗性基因（blaPDC-11、sul1等），以及负责粘附（鞭毛、IV型毛毛）、酶（磷脂酶C）、铁摄取（pyoverdine）、分泌系统（exoT、exoU）和毒素（toxA）分泌的毒力基因。因此，城市污水是耐多药铜绿假单胞菌的潜在宿主，建议在主要污染源建立污水处理厂（WWTPs），然后将其排放到废水网络中，以降低暴发的风险。

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来源期刊

World journal of microbiology & biotechnology 工程技术-生物工程与应用微生物

CiteScore

6.30

自引率

2.40%

发文量

257

审稿时长

2.5 months

期刊介绍： World Journal of Microbiology and Biotechnology publishes research papers and review articles on all aspects of Microbiology and Microbial Biotechnology. Since its foundation, the Journal has provided a forum for research work directed toward finding microbiological and biotechnological solutions to global problems. As many of these problems, including crop productivity, public health and waste management, have major impacts in the developing world, the Journal especially reports on advances for and from developing regions. Some topics are not within the scope of the Journal. Please do not submit your manuscript if it falls into one of the following categories: · Virology · Simple isolation of microbes from local sources · Simple descriptions of an environment or reports on a procedure · Veterinary, agricultural and clinical topics in which the main focus is not on a microorganism · Data reporting on host response to microbes · Optimization of a procedure · Description of the biological effects of not fully identified compounds or undefined extracts of natural origin · Data on not fully purified enzymes or procedures in which they are applied All articles published in the Journal are independently refereed.