Deciphering the regulatory network of lignocellulose biosynthesis in bread wheat through genome-wide association studies.

Abstract

Key message: This study identified 46 key QTL and 17 candidate genes and developed a KASP marker, providing valuable molecular tools for enhancing lignocellulose traits, lodging resistance, and bioenergy potential in wheat. Wheat lignocellulose, composed of lignin, cellulose, and hemicellulose, plays a crucial role in strengthening plant cell walls, enhancing lodging resistance, and contributing to bioenergy production. However, the genetic basis underlying the variation in lignocellulose content in wheat remains poorly understood. The stem lignin, cellulose, and hemicellulos contents in the second stem internode of a panel of 166 wheat accessions grown in three environments were measured, combined with the genotyping data with 660 K wheat SNP chip; a genome-wide association studies (GWAS) were conducted to identify loci associated with the lignocellulose content in wheat. Significant variations in lignin, cellulose, and hemicellulose contents were observed among the wheat accessions. GWAS identified 1146 significant SNPs associated with lignin, cellulose, and hemicellulose contents, distributed across the A, B, and D sub-genomes of wheat. Joint analysis of haplotype blocks refined these associations, identifying 46 significant quantitative trait loci (QTL) regions and 17 candidate genes, primarily linked to vascular development, hemicellulose synthesis, internode elongation regulation, and lignin biosynthesis. A KASP marker (NW_CC5951) for lignocellulose was developed. These findings provide valuable molecular markers for marker-assisted selection, supporting wheat breeding for improved stem quality and lodging resistance, and offer insights into balancing grain yield with lodging resistance and lignocellulosic energy production.