Cristian Falero, Wilfredo Huanca, Luis Barrios-Arpi, Boris Lira-Mejía, Olger Ramos-Coaguila, Edith Torres, Eva Ramos, Alejandro Romero, Mariella Ramos-Gonzalez
{"title":"Oxidative and Molecular-Structural Alterations of Spermatozoa in Swine and Ram Exposed to the Triazole Ipconazole.","authors":"Cristian Falero, Wilfredo Huanca, Luis Barrios-Arpi, Boris Lira-Mejía, Olger Ramos-Coaguila, Edith Torres, Eva Ramos, Alejandro Romero, Mariella Ramos-Gonzalez","doi":"10.3390/toxics13030176","DOIUrl":null,"url":null,"abstract":"<p><p>Triazole pesticides are widely used throughout the world, but their abuse causes toxic effects in non-targeted organisms. In the present study, the cytotoxic effect of the triazole ipconazole was evaluated in porcine and ram spermatozoa. Ipconazole significantly reduced sperm viability, increased ROS levels, altered catalase and SOD enzyme activity, and caused alterations in the molecular mRNA expression of structural biomarkers (PRM1, ODF2, AKAP4, THEG, SPACA3 and CLGN) related to fertility in males, as well as the overexpression of BAX (cell death) and ROMO1 (oxidative stress) mRNA. Our results indicate that the fungicide triazole is involved in cellular, enzymatic and molecular alteration of porcine and ram spermatozoa, and is possibly a factor in the development of infertility in male mammals.</p>","PeriodicalId":23195,"journal":{"name":"Toxics","volume":"13 3","pages":""},"PeriodicalIF":3.9000,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11945538/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Toxics","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.3390/toxics13030176","RegionNum":3,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Triazole pesticides are widely used throughout the world, but their abuse causes toxic effects in non-targeted organisms. In the present study, the cytotoxic effect of the triazole ipconazole was evaluated in porcine and ram spermatozoa. Ipconazole significantly reduced sperm viability, increased ROS levels, altered catalase and SOD enzyme activity, and caused alterations in the molecular mRNA expression of structural biomarkers (PRM1, ODF2, AKAP4, THEG, SPACA3 and CLGN) related to fertility in males, as well as the overexpression of BAX (cell death) and ROMO1 (oxidative stress) mRNA. Our results indicate that the fungicide triazole is involved in cellular, enzymatic and molecular alteration of porcine and ram spermatozoa, and is possibly a factor in the development of infertility in male mammals.
ToxicsChemical Engineering-Chemical Health and Safety
CiteScore
4.50
自引率
10.90%
发文量
681
审稿时长
6 weeks
期刊介绍：
Toxics (ISSN 2305-6304) is an international, peer-reviewed, open access journal which provides an advanced forum for studies related to all aspects of toxic chemicals and materials. It publishes reviews, regular research papers, and short communications. Our aim is to encourage scientists to publish their experimental and theoretical results in detail. There is, therefore, no restriction on the maximum length of the papers, although authors should write their papers in a clear and concise way. The full experimental details must be provided so that the results can be reproduced. Electronic files or software regarding the full details of calculations and experimental procedure can be deposited as supplementary material, if it is not possible to publish them along with the text.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
