Detection of hepatitis delta virus RNA in cervical swab and seminal plasma of chronic hepatitis delta patients.

IF 3.6 3区医学 Q2 INFECTIOUS DISEASES

Sexually Transmitted Infections Pub Date : 2025-03-27 DOI:10.1136/sextrans-2024-056364

Saruul Enkhjargal, Oyungerel Lhagva-Ochir, Altangerel Purvee, Galtbayar Artbazar, Maria Paola Anolli, Anir Enkhbat, Nomin Ariungerel, Maral Enkhtur, Oyungerel Ravjir, Nyamtsengel Vangan, Naranjargal Dashdorj, Odgerel Oidovsambuu

{"title":"Detection of hepatitis delta virus RNA in cervical swab and seminal plasma of chronic hepatitis delta patients.","authors":"Saruul Enkhjargal, Oyungerel Lhagva-Ochir, Altangerel Purvee, Galtbayar Artbazar, Maria Paola Anolli, Anir Enkhbat, Nomin Ariungerel, Maral Enkhtur, Oyungerel Ravjir, Nyamtsengel Vangan, Naranjargal Dashdorj, Odgerel Oidovsambuu","doi":"10.1136/sextrans-2024-056364","DOIUrl":null,"url":null,"abstract":"Objectives: Hepatitis delta virus (HDV) is the smallest human virus that causes the most severe form of viral hepatitis. However, the virus's biological features, transmission routes and causes of endemicity in particular populations are not yet fully understood. HDV shares the hepatitis B virus (HBV) parenteral mode of transmission with HBV; therefore, sexual transmissions are also possible. However, data on sexual transmission of HDV are limited.Methods: This study included 60 patients with chronic HBV/HDV coinfection. The median age (IQR) of patients was 35 (23-47) years, and all were Mongolian. HDV-RNA, HBV-DNA and hepatitis B surface antigens were tested in serum, seminal plasma and cervical swab samples collected at the Liver Centre, Mongolia. Binary logistic regression analysis was used to create a model that predicts the possibility of HDV detection in seminal plasma and cervical swab samples based on HDV-RNA levels in serum samples.Results: HDV-RNA was detected in cervical swab samples from 24 of 30 patients (80%) and in seminal plasma from 15 of 30 patients (50%) (Fisher's exact test, p=0.03). Patients with detectable HDV-RNA in either seminal or cervical fluid samples showed higher levels of HDV-RNA in serum ((females: 6.44 vs 4.21 log IU/mL (p<0.0001); males: 6.69 vs 5.65 log IU/mL (p<0.0001)). A correlation between HDV-RNA levels in the serum was identified in females (r=0.56, p=0.004; 95% CI 0.206 to 0.788) but not in males (r=0.38, p=0.15, 95% CI -0.15 to 0.75). The regression model identified threshold points of serum HDV-RNA levels that can be used to predict HDV-RNA detection in seminal plasma and cervical swabs.Conclusions: HDV-RNA can be detected in cervical swabs and seminal plasma of patients with HBV/HDV coinfection, and the detection was more common in females than in males. The probability of HDV-RNA detection in male seminal plasma and female cervical swab samples can be predicted based on the HDV viral load in serum.","PeriodicalId":21624,"journal":{"name":"Sexually Transmitted Infections","volume":" ","pages":""},"PeriodicalIF":3.6000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sexually Transmitted Infections","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1136/sextrans-2024-056364","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives: Hepatitis delta virus (HDV) is the smallest human virus that causes the most severe form of viral hepatitis. However, the virus's biological features, transmission routes and causes of endemicity in particular populations are not yet fully understood. HDV shares the hepatitis B virus (HBV) parenteral mode of transmission with HBV; therefore, sexual transmissions are also possible. However, data on sexual transmission of HDV are limited.

Methods: This study included 60 patients with chronic HBV/HDV coinfection. The median age (IQR) of patients was 35 (23-47) years, and all were Mongolian. HDV-RNA, HBV-DNA and hepatitis B surface antigens were tested in serum, seminal plasma and cervical swab samples collected at the Liver Centre, Mongolia. Binary logistic regression analysis was used to create a model that predicts the possibility of HDV detection in seminal plasma and cervical swab samples based on HDV-RNA levels in serum samples.

Results: HDV-RNA was detected in cervical swab samples from 24 of 30 patients (80%) and in seminal plasma from 15 of 30 patients (50%) (Fisher's exact test, p=0.03). Patients with detectable HDV-RNA in either seminal or cervical fluid samples showed higher levels of HDV-RNA in serum ((females: 6.44 vs 4.21 log IU/mL (p<0.0001); males: 6.69 vs 5.65 log IU/mL (p<0.0001)). A correlation between HDV-RNA levels in the serum was identified in females (r=0.56, p=0.004; 95% CI 0.206 to 0.788) but not in males (r=0.38, p=0.15, 95% CI -0.15 to 0.75). The regression model identified threshold points of serum HDV-RNA levels that can be used to predict HDV-RNA detection in seminal plasma and cervical swabs.

Conclusions: HDV-RNA can be detected in cervical swabs and seminal plasma of patients with HBV/HDV coinfection, and the detection was more common in females than in males. The probability of HDV-RNA detection in male seminal plasma and female cervical swab samples can be predicted based on the HDV viral load in serum.

查看原文本刊更多论文

慢性丁型肝炎患者宫颈拭子和精浆中丁型肝炎病毒RNA的检测。

目的：丁型肝炎病毒（HDV）是最小的人类病毒，可引起最严重的病毒性肝炎。然而，该病毒的生物学特征、传播途径和在特定人群中流行的原因尚未完全了解。HDV与HBV共享乙型肝炎病毒（HBV）的肠外传播方式；因此，性传播也是可能的。然而，关于HDV性传播的数据有限。方法：本研究纳入60例慢性HBV/HDV合并感染患者。患者中位年龄（IQR）为35（23-47）岁，均为蒙古族患者。在蒙古肝脏中心采集的血清、精浆和宫颈拭子样本中检测了hiv - rna、HBV-DNA和乙型肝炎表面抗原。采用二元logistic回归分析建立了一个基于血清样本中HDV- rna水平预测精浆和宫颈拭子样本中HDV检测可能性的模型。结果：在30例患者的宫颈拭子样本中检测到HDV-RNA(80%)，在30例患者的精浆中检测到15例（50%）（Fisher精确检验，p=0.03）。在精液和宫颈液中检测到HDV- rna的患者血清中HDV- rna水平较高（女性：6.44 vs 4.21 log IU/mL）。结论：HBV/HDV合并感染患者的宫颈棉子和精液中均可检测到HDV- rna，且女性比男性更常见。男性精浆和女性宫颈拭子样本中检测到HDV- rna的概率可根据血清中HDV病毒载量进行预测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Sexually Transmitted Infections 医学-传染病学

CiteScore

5.70

自引率

8.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Sexually Transmitted Infections is the world’s longest running international journal on sexual health. It aims to keep practitioners, trainees and researchers up to date in the prevention, diagnosis and treatment of all STIs and HIV. The journal publishes original research, descriptive epidemiology, evidence-based reviews and comment on the clinical, public health, sociological and laboratory aspects of sexual health from around the world. We also publish educational articles, letters and other material of interest to readers, along with podcasts and other online material. STI provides a high quality editorial service from submission to publication.