Screening for the ciliate Buxtonella sulcata in free-ranging dairy cattle on Terceira Island, Azores archipelago.

IF 2.3 2区医学 Q2 PARASITOLOGY

Parasite Pub Date : 2025-01-01 Epub Date: 2025-03-28 DOI:10.1051/parasite/2025014

Mário Ribeiro, Sara Gomes-Gonçalves, Alexandra Silva, Guilherme Moreira, Eric Viscogliosi, Magali Chabé, João Rodrigo Mesquita

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Abstract

Buxtonella sulcata is an alveolate ciliate protist, historically considered a commensal of the gastrointestinal tract of cattle. Despite the fact that its cysts are morphologically identical to those of Balantioides coli, molecular identification techniques have shed new light on its role as a pathogen. This work aimed to assess the presence of this ciliate in the population of dairy cattle on the Azorean island of Terceira by means of molecular analyses (ITS1-5.8S-ITS2 rRNA) of stool samples. A total of 116 samples were collected from adult Holstein-Friesian dairy cows, with no signs of gastrointestinal disease. A proportion of 49.1% of the samples were PCR-positive for Bu. sulcata, and 12 different genetic sequences were identified. These findings highlight the need for future research concerning the factors that influence the presence of Bu. sulcata in the gastrointestinal tract of dairy cows, the role of bovines as possible sources of infection, and the impact this ciliate may have on the health, welfare, and productivity of these animals.

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亚速尔群岛特塞拉岛散养奶牛中纤毛虫短孔虫的筛查。

沟巴克通体是一种肺泡状纤毛虫原生生物，历来被认为是牛胃肠道的共生菌。尽管它的囊肿在形态上与大肠杆菌的囊肿相同，但分子鉴定技术已经为其作为病原体的作用提供了新的线索。本工作旨在通过粪便样本的分子分析（ITS1-5.8S-ITS2 rRNA）来评估该纤毛虫在亚速尔群岛特塞拉岛奶牛种群中的存在。从没有胃肠道疾病迹象的成年荷斯坦-弗里西亚奶牛中共收集了116个样本。49.1%的标本pcr阳性。Sulcata和12个不同的基因序列。这些发现强调了对影响Bu存在的因素进行进一步研究的必要性。乳牛胃肠道中的纤毛虫，牛作为可能感染源的作用，以及这种纤毛虫可能对这些动物的健康、福利和生产力产生的影响。

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来源期刊

Parasite 医学-寄生虫学

CiteScore

5.50

自引率

6.90%

发文量

审稿时长

3 months

期刊介绍： Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.